Western blot analysis of lysates from NIH/3T3 cells usingย Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (STJ110835) atย 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 ยฐC for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25ย  Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time:ย 180s.

Anti-Phospho-MAPK1/MAPK3-T202/Y204 & T185/Y187 antibody (STJ110835)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110835
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General Information

Short DescriptionRabbit polyclonal Phospho-MAPK1/MAPK3-T202/Y204 & T185/Y187 antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMAPK3
MAPK1
Gene ID5595
5594
Uniprot IDMK03_HUMAN
MK01_HUMAN
Immunogen SequenceLTEYV
SpecificityA synthetic phosphorylated peptide around T185 & Y187 of human ERK2MAPK1 (NP_002736.3).

Additional Info

Tissue Specificity
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 3 antibody
Anti-Map Kinase 3 antibody
Anti-Mapk 3 antibody
Anti-Ert2 antibody
Anti-Extracellular Signal-Regulated Kinase 1 antibody
Anti-Erk-1 antibody
Anti-Insulin-Stimulated Map2 Kinase antibody
Anti-Map Kinase Isoform P44 antibody
Anti-P44-Mapk antibody
Anti-Microtubule-Associated Protein 2 Kinase antibody
Anti-P44-Erk1 antibody
Anti-MAPK3 antibody
Anti-ERK1 antibody
Anti-PRKM3 antibody
Anti-Mitogen-Activated Protein Kinase 1 antibody
Anti-Map Kinase 1 antibody
Anti-Mapk 1 antibody
Anti-Ert1 antibody
Anti-Extracellular Signal-Regulated Kinase 2 antibody
Anti-Erk-2 antibody
Anti-Map Kinase Isoform P42 antibody
Anti-P42-Mapk antibody
Anti-Mitogen-Activated Protein Kinase 2 antibody
Anti-Map Kinase 2 antibody
Anti-Mapk 2 antibody
Anti-MAPK1 antibody
Anti-ERK2 antibody
Anti-PRKM1 antibody
Anti-PRKM2 antibody

Information sourced from Uniprot.org

Citations

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