Anti-Phospho-MECP2-Ser-80 antibody (STJA0005464)

SKU:
STJA0005464-100

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Host: Rabbit
Applications: WB/ICC/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-MeCP2-Ser-80 is suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Antigen Affinity Purified
Dilution Range: WB 1:1000
IHC 1:100
ICC 1:250
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mecp2
Gene ID: 17257
Uniprot ID: MECP2_MOUSE
Specificity: The antibody detects a 75 kDa* protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment.
Immunogen: phospho-MeCP2 (Ser-80) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 80 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins.
Post Translational Modifications Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. Does not seem to be phosphorylated on Ser-421 in other tissues.
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Protein Name Methyl-Cpg-Binding Protein 2
Mecp-2 Protein
Mecp2
Database Links Reactome: R-MMU-9022692 Q9Z2D6-1
Cellular Localisation Nucleus
Colocalized With Methyl-Cpg In The Genome
Colocalized With Tbl1x To The Heterochromatin Foci
Alternative Antibody Names Anti-Methyl-Cpg-Binding Protein 2 antibody
Anti-Mecp-2 Protein antibody
Anti-Mecp2 antibody
Anti-Mecp2 antibody

Information sourced from Uniprot.org

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