Anti-Phospho-MeCP2-Ser-80 antibody (STJA0041281)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
Immunogenphospho-MeCP2 (Ser-80) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 80 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins.
General Information
| Short Description | Rabbit polyclonal anti-Phospho-MeCP2-Ser-80 for use in ELISA, WB, ICC and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/ICC/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000IHC 1:100ICC 1:250 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Storage at-20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at-20°C. |
Target Information
| Gene Symbol | Mecp2 |
| Gene ID | 17257 |
| Uniprot ID | MECP2_MOUSE |
| Immunogen | phospho-MeCP2 (Ser-80) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 80 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins. |
| Immunogen Sequence | Mouse |
| Specificity | The antibody detects a 75 kDa* protein corresponding to the molecular mass of MeCP2 on SDS-PAGE immunoblots of human PC3 cells treated with calyculin A and in mouse brain tissue. These reactivities are not observed after lambda phosphatase treatment. |
Additional Info
| Post Translational Modifications | Phosphorylated on Ser-421 by CaMK2 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. Does not seem to be phosphorylated on Ser-421 in other tissues. |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). |
| Protein Name | Methyl-Cpg-Binding Protein 2Mecp-2 ProteinMecp2 |
| Database Links | Reactome: R-MMU-9022692 |
| Cellular Localisation | NucleusColocalized With Methyl-Cpg In The GenomeColocalized With Tbl1x To The Heterochromatin Foci |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Protein 2 antibodyAnti-Mecp-2 Protein antibodyAnti-Mecp2 antibodyAnti-Mecp2 antibody |
Information sourced from Uniprot.org