ELISA Antibodies | St John's Laboratory

Western blot analysis of lysates from HeLa cells, using ATF4 Rabbit polyclonal antibody (STJ22704) at 1:1000 dilution. HeLa cells were treated by tunicamycin (2 Mu g/mL) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-ATF4 antibody (1-351) (STJ22704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of A549 cells using CREB-2 Polyclonal Antibody

Anti-ATF4 antibody (160-240 aa) (STJ92467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of Hep G2 cells using ATF4 Rabbit polyclonal antibody (STJ11101481) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATF4 antibody (260-351) (STJ11101481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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STJ70090 (1µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ATF4 antibody (C-Term) (STJ70090)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Pig

$431.25

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Immunofluorescence analysis of U2OS cells using ATF5 Rabbit polyclonal antibody (STJ11100125) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATF5 antibody (101-282) (STJ11100125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry analysis of paraffin-embedded human placenta using ATF5 Rabbit monoclonal antibody (STJ11101898) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-ATF5 antibody (183-282) [S8MR] (STJ11101898)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of various cells using ATF-5 Polyclonal Antibody diluted at 1：1000

Anti-ATF5 antibody (221-270 aa) (STJ91753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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STJ70089 (1. 5µg/ml) staining of Human Heart lyssate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ATF5 antibody (C-Term) (STJ70089)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

$431.25

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Immunofluorescence analysis of PC-12 cells using ATF6 Rabbit polyclonal antibody (STJ22705) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATF6 antibody (190-670) (STJ22705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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EB012080 (1µg/ml) staining of HeLa (A) and MCF7 (B) cell lysate, and HeLa (C) and Jurkat (D) nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ATF6 antibody (437-449) (STJ72994)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Rat/Dog/Pig/Cow

$431.25

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Immunofluorescence analysis of PC-12 cells using ATF6 Rabbit polyclonal antibody (STJ11103832) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATF6 antibody (600-670) (STJ11103832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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STJ73653 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ATF6 antibody (C or N-Term) (STJ73653)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

$431.25

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Anti-ATF6 antibody [SRM15045] (STJ11109775)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IHC-P/ELISA

Reactivity :

Human

$0.00

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Anti-ATF6A antibody [PT0177R] (STJA0039729)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Western blot analysis of various cells using ATF-6 Beta Polyclonal Antibody

Anti-ATF6B antibody (401-450 aa) (STJ91754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using ATF7 antibody (STJ29717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATF7 antibody (1-117) (STJ29717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of various cells using ATF-7 Polyclonal Antibody

Anti-ATF7 antibody (41-90 aa) (STJ91755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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STJ70088 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ATF7 antibody (C-Term) (STJ70088)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Rat/Dog/Cow

$431.25

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Anti-ATF7 antibody [SAA2118] (STJA0022319)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

$522.33 - $2,252.85

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Anti-ATF7 antibody [SAA2119] (STJA0022320)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

$522.33 - $2,252.85

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Western blot analysis of extracts of various cell lines, using ATF7IP antibody (STJ116407) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ATF7IP antibody (525-725) (STJ116407)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200

Anti-ATG10 antibody (1-50 aa) (STJ98826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of NIH-3T3 cells using ATG10 Rabbit monoclonal antibody (STJ11102331) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG10 antibody (121-220) [S1MR] (STJ11102331)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Immunohistochemistry analysis of paraffin-embedded Rat testis using ATG10 Rabbit polyclonal antibody (STJ29527) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ATG10 antibody (204-220) (STJ29527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of U2OS cells using ATG12 Rabbit polyclonal antibody (STJ118058) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG12 antibody (1-100) (STJ118058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using ATG12 antibody (STJ11101729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ATG12 antibody (41-140) [S9MR] (STJ11101729)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-ATG12 antibody [SRM13260] (STJ11107990)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

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Anti-ATG13 antibody (281-480 aa) [SRM5259] (STJ11107161)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $765.21

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Western blot analysis of various lysates, using ATG13 antibody (STJ22706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 25s.

Anti-ATG13 antibody (281-517) (STJ22706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-ATG13 antibody [PT1022R] (STJA0039947)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunofluorescence analysis of U2OS cells using ATG14 Rabbit polyclonal antibody (STJ29664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG14 antibody (340-440) (STJ29664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-ATG14 antibody [PT1393R] (STJA0040026)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunofluorescence analysis of U2OS cells using ATG16L1 Rabbit polyclonal antibody (STJ113881) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ATG16L1 antibody (200-300) (STJ113881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry of rat tissue with Anti-ATG16L1 primary antibody (STJ500180) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 5X.

Anti-ATG16L1 antibody (560-610) (STJ500180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$589.95

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Anti-ATG16L1 antibody (560-610) {Biotin} (STJ500181)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Anti-ATG16L1 antibody (560-610) {FITC} (STJ500182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$729.33

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Western blot analysis of extracts of various cell lines, using ATG16L1 Rabbit monoclonal antibody (STJ11101913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATG16L1 antibody (6-105) [S3MR] (STJ11101913)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Western blot analysis of Jurkat using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-ATG16L1 antibody (60-140 aa) (STJ91756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of A-549 cells using ATG16L1 antibody (STJ22707). Blue: DAPI for nuclear staining.

Anti-ATG16L1 antibody (90-300) (STJ22707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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STJ71227 (0. 1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ATG16L1 antibody (Internal) (STJ71227)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

$431.25

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Anti-ATG16L1 antibody [PT0690R] (STJA0039266)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Western blot analysis of various lysates using ATG16L2 Rabbit polyclonal antibody (STJ117147) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATG16L2 antibody (1-275) (STJ117147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of ATG16L2 Antibody. The lane on the right is blocked with the ATG16L2 peptide.

Anti-ATG16L2 antibody (241-290 aa) (STJ91757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates using ATG2A Rabbit pAb (STJ111311) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ATG2A antibody (700-800) (STJ111311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of various lysates using ATG2B Rabbit pAb (STJ110796) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-ATG2B antibody (1360-1600) (STJ110796)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using ATG3 antibody (STJ11101718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-ATG3 antibody (1-100) [S8MR] (STJ11101718)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunofluorescence analysis of NIH-3T3 cells using ATG3 Rabbit polyclonal antibody (STJ28372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG3 antibody (1-314) (STJ28372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-ATG3 antibody [PT0538R] (STJA0039310)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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