CLIA Kits

Kits

Chemiluminescent Immunoassay (CLIA) Kits

Sandwich-format CLIA kits validated across human, mouse and rat targets.

Our CLIA kits use the same sandwich capture format as our ELISA range, but the target is detected with light instead of colour. Once the reaction runs, a luminometer reads the light output as relative light units (RLU), and analyte concentration is worked out from that reading instead of an optical density measurement.

Category benefits

Why use CLIA over colorimetric ELISA?

✓ Greater sensitivity
Chemiluminescent detection can resolve analyte concentrations down to roughly 10⁻¹⁶ mol/L, into the sub-femtomolar range, lower than colorimetric ELISA typically distinguishes from background.
✓ Wider dynamic range
The relationship between analyte concentration and light output holds across a broader span of concentrations than immunoenzymatic (ELISA) formats, useful when a sample's analyte level is not known in advance.
✓ Lower background signal
Samples produce little or no native chemiluminescence of their own, so the background reading is lower than with absorbance-based detection.
Where they're used

Typical applications

✓ Low-abundance cytokine and hormone quantification
The sensitivity of chemiluminescent detection suits cytokines, hormones and other analytes present at low concentrations in serum, plasma and other biological fluids.
✓ Samples with an unknown concentration range
The wider dynamic range means a single assay can measure both low and high analyte levels without pre-diluting or re-running samples at multiple dilutions.
✓ Assay development and ELISA transition
Where a colorimetric ELISA is reaching the lower limit of what it can distinguish from background, moving the same capture and detection antibody pair to chemiluminescent readout extends the assay's usable range.

Need help choosing a CLIA kit?

Our technical team can help match a target, species and sample type to the right kit. Each kit's own datasheet lists its confirmed sensitivity, dynamic range and cross-reactivity data.

Looking for more kits?

Browse our full kit range, spanning CLIA, ELISA and antibody pair formats.

 Browse all kits

For Research Use Only. Not for use in diagnostic procedures.

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