Human ANGPT4 (Angiopoietin-4) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human ANGPT4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 18.75pg/mL |
| Detection Limit | 31.25~2000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | ANGPT4 |
| Gene ID | 51378 |
| Uniprot ID | ANGP4_HUMAN |
| Specificity | This kit recognizes Human ANGPT4 in samples. No significant cross-reactivity or interference between Human ANGPT4 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Highly expressed in the lung with much lower levels found in other tissues. |
| Function | Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis. |
| Protein Name | Angiopoietin-4Ang-4Angiopoietin-3Ang-3 |
| Database Links | Reactome: R-HSA-210993 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Angiopoietin-4 CLIA kitAng-4 CLIA kitAngiopoietin-3 CLIA kitAng-3 CLIA kitANGPT4 CLIA kitANG3 CLIA kitANG4 CLIA kit |
| Specificity | This kit recognizes Human ANGPT4 in samples. No significant cross-reactivity or interference between Human ANGPT4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 98-112 | 104 |
| EDTA plasma(n=8) | 89-101 | 94 |
| Cell culture media(n=8) | 100-116 | 106 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 102.86 | 300.31 | 973.57 | 99.81 | 291.76 | 1051.18 |
| Standard deviation | 11.51 | 28.50 | 62.60 | 11.26 | 23.31 | 87.88 |
| CV (%) | 11.19 | 9.49 | 6.43 | 11.28 | 7.99 | 8.36 |