Human CCL17 (C-C motif chemokine 17) CLIA Kit for Quantitative Detection
General Information
| Short Description | This human CCL17 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 7.5pg/mL |
| Detection Limit | 12.5~800pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | CCL17 |
| Gene ID | 6361 |
| Uniprot ID | CCL17_HUMAN |
| Specificity | This kit recognizes Human TARC in samples. No significant cross-reactivity or interference between Human TARC and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Constitutively expressed in thymus. Detected at lower levels in the lung, colon and small intestine. Expressed in stimulated peripheral blood mononuclear cells, but not in resting cells. |
| Function | Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes. Acts by binding to CCR4 at T-cell surface. Mediates GM-CSF/CSF2-driven pain and inflammation. In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation. Plays a role in wound healing, mainly by inducing fibroblast migration into the wound. |
| Protein Name | C-C Motif Chemokine 17Cc Chemokine TarcSmall-Inducible Cytokine A17Thymus And Activation-Regulated Chemokine |
| Database Links | Reactome: R-HSA-380108 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-C Motif Chemokine 17 CLIA kitCc Chemokine Tarc CLIA kitSmall-Inducible Cytokine A17 CLIA kitThymus And Activation-Regulated Chemokine CLIA kitCCL17 CLIA kitSCYA17 CLIA kitTARC CLIA kit |
| Specificity | This kit recognizes Human TARC in samples. No significant cross-reactivity or interference between Human TARC and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-105 | 98 |
| EDTA plasma(n=8) | 86-99 | 92 |
| Cell culture media(n=8) | 91-108 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 41.32 | 106.81 | 382.44 | 39.32 | 109.52 | 411.93 |
| Standard deviation | 3.50 | 7.88 | 29.95 | 3.86 | 8.62 | 38.89 |
| CV (%) | 8.47 | 7.38 | 7.83 | 9.82 | 7.87 | 9.44 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human CCL17 (C-C motif chemokine 17) CLIA Kit for Quantitative Detection
This kit is designed to quantitatively detect human CCL17, also known as TARC, in serum, plasma, and other biological fluids. It is validated for use with human samples only.
This kit has a detection range of 12.5 to 800 pg/mL and a sensitivity of 7.5 pg/mL. Samples with analyte concentrations outside this range may require dilution or concentration prior to testing.
This kit uses chemiluminescence (CLIA) for signal detection. This method is suited for quantitative measurement of CCL17 in biological fluid samples.
The total assay time is approximately 3.5 hours from start to result.
No significant cross-reactivity or interference between human CCL17 (TARC) and its analogues was observed, based on the specificity data provided for this kit.
If unopened, the kit may be stored at 2 to 8 degrees Celsius for up to one month. If the kit will not be used within one month of receipt, components should be stored separately according to the conditions specified in the manual.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.