Human CASP1 (Caspase-1) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human CASP1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 3.75pg/mL |
| Detection Limit | 6.25~400pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | CASP1 |
| Gene ID | 834 |
| Uniprot ID | CASP1_HUMAN |
| Specificity | This kit recognizes Human CASP1 in samples. No significant cross-reactivity or interference between Human CASP1 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. |
| Post Translational Modifications | The two subunits are derived from the precursor sequence by an autocatalytic mechanism. Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8. Cleavage in the interdomain linker region is required to induce pyroptosis. |
| Function | Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukin IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly. Isoform Delta: Apoptosis inactive. Isoform Epsilon: Apoptosis inactive. |
| Protein Name | Caspase-1Casp-1Interleukin-1 Beta ConvertaseIl-1bcInterleukin-1 Beta-Converting EnzymeIceIl-1 Beta-Converting EnzymeP45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 |
| Database Links | Reactome: R-HSA-168638Reactome: R-HSA-448706Reactome: R-HSA-5620971Reactome: R-HSA-6803207Reactome: R-HSA-844456Reactome: R-HSA-844615Reactome: R-HSA-844623Reactome: R-HSA-9008059Reactome: R-HSA-9660826Reactome: R-HSA-9692916 |
| Cellular Localisation | CytoplasmCell Membrane |
| Alternative CLIA Names | Caspase-1 CLIA kitCasp-1 CLIA kitInterleukin-1 Beta Convertase CLIA kitIl-1bc CLIA kitInterleukin-1 Beta-Converting Enzyme CLIA kitIce CLIA kitIl-1 Beta-Converting Enzyme CLIA kitP45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 CLIA kitCASP1 CLIA kitIL1BC CLIA kitIL1BCE CLIA kit |
| Specificity | This kit recognizes Human CASP1 in samples. No significant cross-reactivity or interference between Human CASP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-105 | 96 |
| EDTA plasma(n=8) | 90-104 | 97 |
| Cell culture media(n=8) | 93-106 | 101 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 18.81 | 49.91 | 189.93 | 18.27 | 45.54 | 182.67 |
| Standard deviation | 1.63 | 5.07 | 16.77 | 1.79 | 5.41 | 19.55 |
| CV (%) | 8.67 | 10.16 | 8.83 | 9.80 | 11.88 | 10.70 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human CASP1 (Caspase-1) CLIA Kit for Quantitative Detection
This kit is designed to quantitatively detect Human CASP1 (Caspase-1) in human serum, plasma, and other biological fluids. No significant cross-reactivity or interference with Human CASP1 analogues was observed.
This kit has a detection range of 6.25 to 400 pg/mL and a sensitivity of 3.75 pg/mL.
This kit uses chemiluminescence immunoassay (CLIA) for quantitative detection of Human CASP1.
The total assay time is approximately 3.5 hours.
If unopened, this kit can be stored at 2 to 8 degrees Celsius for up to 1 month. If the kit will not be used within 1 month, components should be stored separately according to the component table in the manual.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.