Human BPI (Bactericidal permeability-increasing protein) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human BPI kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 7.5pg/mL |
| Detection Limit | 12.5~800pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | BPI |
| Gene ID | 671 |
| Uniprot ID | BPI_HUMAN |
| Specificity | This kit recognizes Human BPI in samples. No significant cross-reactivity or interference between Human BPI and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Restricted to cells of the myeloid series. |
| Function | The cytotoxic action of BPI is limited to many species of Gram-negative bacteria.this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. |
| Protein Name | Bactericidal Permeability-Increasing ProteinBpiCap 57 |
| Database Links | Reactome: R-HSA-166016Reactome: R-HSA-6798695Reactome: R-HSA-6803157 |
| Cellular Localisation | SecretedCytoplasmic Granule MembraneMembrane-Associated In Polymorphonuclear Leukocytes (Pmn) Granules |
| Alternative CLIA Names | Bactericidal Permeability-Increasing Protein CLIA kitBpi CLIA kitCap 57 CLIA kitBPI CLIA kit |
| Specificity | This kit recognizes Human BPI in samples. No significant cross-reactivity or interference between Human BPI and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-106 | 98 |
| EDTA plasma(n=8) | 91-102 | 97 |
| Cell culture media(n=8) | 93-106 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 38.22 | 95.59 | 360.56 | 34.45 | 90.49 | 343.69 |
| Standard deviation | 4.35 | 6.74 | 22.21 | 4.47 | 10.41 | 38.60 |
| CV (%) | 11.38 | 7.05 | 6.16 | 12.98 | 11.50 | 11.23 |