Human APOH (Beta-2-glycoprotein 1) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human APOH kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 46.88pg/mL |
| Detection Limit | 78.13~5000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | APOH |
| Gene ID | 350 |
| Uniprot ID | APOH_HUMAN |
| Specificity | This kit recognizes Human ApoH in samples. No significant cross-reactivity or interference between Human ApoH and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed by the liver and secreted in plasma. |
| Post Translational Modifications | N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele. |
| Function | Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. |
| Protein Name | Beta-2-Glycoprotein 1Apc InhibitorActivated Protein C-Binding ProteinAnticardiolipin CofactorApolipoprotein HApo-HBeta-2-Glycoprotein IB2gpiBeta(2gpi |
| Database Links | Reactome: R-HSA-114608 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Beta-2-Glycoprotein 1 CLIA kitApc Inhibitor CLIA kitActivated Protein C-Binding Protein CLIA kitAnticardiolipin Cofactor CLIA kitApolipoprotein H CLIA kitApo-H CLIA kitBeta-2-Glycoprotein I CLIA kitB2gpi CLIA kitBeta(2gpi CLIA kitAPOH CLIA kitB2G1 CLIA kit |
| Specificity | This kit recognizes Human ApoH in samples. No significant cross-reactivity or interference between Human ApoH and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-101 | 94 |
| EDTA plasma(n=8) | 88-100 | 93 |
| Cell culture media(n=8) | 94-111 | 102 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 247.79 | 574.87 | 1815.52 | 242.73 | 613.72 | 1782.40 |
| Standard deviation | 30.08 | 59.44 | 204.79 | 26.34 | 45.11 | 159.35 |
| CV (%) | 12.14 | 10.34 | 11.28 | 10.85 | 7.35 | 8.94 |