Human BMP4 (Bone morphogenetic protein 4) CLIA Kit for Quantitative Detection
General Information
| Short Description | This human BMP4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 9.38pg/mL |
| Detection Limit | 15.63~1000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | BMP4 |
| Gene ID | 652 |
| Uniprot ID | BMP4_HUMAN |
| Specificity | This kit recognizes Human BMP-4 in samples. No significant cross-reactivity or interference between Human BMP-4 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. |
| Function | Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A. In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Positively regulates the expression of odontogenic development regulator MSX1 via inducing the IPO7-mediated import of SMAD1 to the nucleus. Required for MSX1-mediated mesenchymal molar tooth bud development beyond the bud stage, via promoting Wnt signaling. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling. Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway. Can also signal through non-canonical BMP pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades. For example, induces SRC phosphorylation which, in turn, activates VEGFR2, leading to an angiogenic response. |
| Protein Name | Bone Morphogenetic Protein 4Bmp-4Bone Morphogenetic Protein 2bBmp-2b |
| Database Links | Reactome: R-HSA-2129379Reactome: R-HSA-381426Reactome: R-HSA-8957275Reactome: R-HSA-9754189Reactome: R-HSA-9758920Reactome: R-HSA-9761174Reactome: R-HSA-9793380Reactome: R-HSA-9827857Reactome: R-HSA-9830364Reactome: R-HSA-9830674Reactome: R-HSA-9834899 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative CLIA Names | Bone Morphogenetic Protein 4 CLIA kitBmp-4 CLIA kitBone Morphogenetic Protein 2b CLIA kitBmp-2b CLIA kitBMP4 CLIA kitBMP2B CLIA kitDVR4 CLIA kit |
| Specificity | This kit recognizes Human BMP-4 in samples. No significant cross-reactivity or interference between Human BMP-4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 89-103 | 95 |
| EDTA plasma(n=8) | 96-114 | 104 |
| Cell culture media(n=8) | 91-105 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 55.04 | 148.59 | 372.42 | 55.23 | 146.01 | 388.64 |
| Standard deviation | 6.02 | 15.29 | 23.87 | 5.04 | 14.86 | 44.42 |
| CV (%) | 10.94 | 10.29 | 6.41 | 9.13 | 10.18 | 11.43 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human BMP4 (Bone morphogenetic protein 4) CLIA Kit for Quantitative Detection
This kit is designed to quantitatively detect human BMP-4 (Bone morphogenetic protein 4, UniProt P12644) in serum, plasma, and other biological fluids. It is validated for human samples only and shows no significant cross-reactivity or interference with BMP-4 analogues.
This kit has a detection range of 15.63 to 1000 pg/mL and a sensitivity of 9.38 pg/mL.
This kit uses chemiluminescence (CLIA) as its detection method for quantitative measurement of human BMP-4.
The total assay time is approximately 3.5 hours from start to finish.
If unopened, the kit may be stored at 2 to 8 degrees Celsius for up to one month. If the kit will not be used within one month, components should be stored separately according to the component table provided in the manual.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.