Human AREG (Amphiregulin) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human AREG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 7.5pg/mL |
| Detection Limit | 12.5~800pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | AREG |
| Gene ID | 374 |
| Uniprot ID | AREG_HUMAN |
| Specificity | This kit recognizes Human AR in samples. No significant cross-reactivity or interference between Human AR and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. |
| Protein Name | AmphiregulinArColorectum Cell-Derived Growth FactorCrdgf |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-177929Reactome: R-HSA-179812Reactome: R-HSA-180292Reactome: R-HSA-180336Reactome: R-HSA-182971Reactome: R-HSA-204005Reactome: R-HSA-212718Reactome: R-HSA-2219530Reactome: R-HSA-5638303Reactome: R-HSA-5673001Reactome: R-HSA-5694530Reactome: R-HSA-6811558Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9009391Reactome: R-HSA-9634638Reactome: R-HSA-9818030 |
| Cellular Localisation | MembraneSingle-Pass Membrane Protein |
| Alternative CLIA Names | Amphiregulin CLIA kitAr CLIA kitColorectum Cell-Derived Growth Factor CLIA kitCrdgf CLIA kitAREG CLIA kitAREGB CLIA kitSDGF CLIA kit |
| Specificity | This kit recognizes Human AR in samples. No significant cross-reactivity or interference between Human AR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 87-101 | 93 |
| EDTA plasma(n=8) | 87-102 | 94 |
| Cell culture media(n=8) | 98-113 | 104 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 37.22 | 101.01 | 333.60 | 35.91 | 110.64 | 342.09 |
| Standard deviation | 4.17 | 11.55 | 35.33 | 4.31 | 10.98 | 29.80 |
| CV (%) | 11.20 | 11.43 | 10.59 | 12.00 | 9.92 | 8.71 |