Human CCL2 (C-C motif chemokine 2) CLIA Kit for Quantitative Detection
General Information
| Short Description | This human CCL2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 4.69pg/mL |
| Detection Limit | 7.81~500pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | CCL2 |
| Gene ID | 6347 |
| Uniprot ID | CCL2_HUMAN |
| Specificity | This kit recognizes Human MCP-1 in samples. No significant cross-reactivity or interference between Human MCP-1 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Expressed in monocytes. |
| Post Translational Modifications | Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. N-Glycosylated. |
| Function | Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. |
| Protein Name | C-C Motif Chemokine 2Hc11Monocyte Chemoattractant Protein 1Monocyte Chemotactic And Activating FactorMcafMonocyte Chemotactic Protein 1Mcp-1Monocyte Secretory Protein JeSmall-Inducible Cytokine A2 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-380994Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-9818026 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | C-C Motif Chemokine 2 CLIA kitHc11 CLIA kitMonocyte Chemoattractant Protein 1 CLIA kitMonocyte Chemotactic And Activating Factor CLIA kitMcaf CLIA kitMonocyte Chemotactic Protein 1 CLIA kitMcp-1 CLIA kitMonocyte Secretory Protein Je CLIA kitSmall-Inducible Cytokine A2 CLIA kitCCL2 CLIA kitMCP1 CLIA kitSCYA2 CLIA kit |
| Specificity | This kit recognizes Human MCP-1 in samples. No significant cross-reactivity or interference between Human MCP-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 100-112 | 106 |
| EDTA plasma(n=8) | 90-104 | 98 |
| Cell culture media(n=8) | 93-107 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 26.97 | 50.59 | 231.90 | 24.71 | 45.93 | 218.62 |
| Standard deviation | 3.42 | 4.44 | 20.06 | 2.94 | 3.69 | 22.06 |
| CV (%) | 12.68 | 8.78 | 8.65 | 11.90 | 8.03 | 10.09 |
Citations
Product Review
Frequently Asked Questions
Common questions about Human CCL2 (C-C motif chemokine 2) CLIA Kit for Quantitative Detection
This kit is designed to quantitatively detect human CCL2, also known as MCP-1 (UniProt P13500), in human serum, plasma, and other biological fluids. It has not been validated for use in non-human species.
This kit has a detection range of 7.81 to 500 pg/mL and a sensitivity of 4.69 pg/mL. Samples with CCL2 concentrations outside the upper limit of this range may require dilution prior to testing.
This kit uses chemiluminescence (CLIA) for signal detection. This method is well suited for quantitative measurement of low-abundance proteins in biological fluids.
The total assay time is approximately 3.5 hours from start to finish.
According to the product specifications, no significant cross-reactivity or interference between human MCP-1 and its analogues was observed, supporting specificity for the intended target.
If unopened, the kit may be stored at 2 to 8 degrees Celsius for up to one month. If the kit will not be used within one month of receipt, components should be stored separately according to the conditions specified in the product manual.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.