Matched Antibody Pairs for Sandwich Immunoassays
Accelerate your immunoassay development with St John's Laboratory's pre-validated Matched Antibody Pairs. Each pair comprises two high-affinity antibodies targeting distinct, non-overlapping epitopes on the same antigen—engineered for optimal capture and detection in sandwich ELISA formats. Skip months of optimisation and deliver reliable, reproducible results from day one.
Why Choose Validated Antibody Pairs?
Pre-Validated Performance
Each pair is rigorously tested for compatibility, ensuring simultaneous binding without steric hindrance or epitope competition.
Time & Cost Savings
Bypass extensive screening and optimisation. Our pairs are ready to use, reducing assay development time from months to days.
High Sensitivity & Specificity
Optimised antibody combinations deliver robust signal-to-noise ratios and minimal cross-reactivity across diverse sample types.
Broad Species Coverage
Extensive selection spanning human, mouse, rat, pig, dog, and more—supporting translational research and comparative studies.
How Matched Antibody Pairs Work
In sandwich immunoassays, success depends on two antibodies recognising separate epitopes on the target antigen without interfering with each other. Our Matched Antibody Pairs are specifically selected and validated to meet this requirement:
Browse All Antibody PairsCapture + Detection Strategy
Capture Antibody: Immobilised on the plate surface to bind and concentrate the target antigen from complex samples (serum, plasma, cell lysates).
Detection Antibody: Recognises a distinct epitope, enabling specific signal generation without competing for the capture site.
Result: Maximum sensitivity, excellent dynamic range, and minimal background—even in unpurified samples.
This dual-recognition approach is the gold standard for quantitative immunoassays, offering superior specificity compared to single-antibody formats.
Featured High-Impact Targets
Explore our most requested antibody pairs for key biomarkers in immunology, inflammation, oncology, and neuroscience:
IFN-γ (Interferon Gamma)
Critical Th1 cytokine for immunology and cancer research. Available for human, mouse, and rat.
IL-6 (Interleukin-6)
Essential inflammation biomarker with broad therapeutic relevance. Available for human, mouse, rat, and pig.
TNF-α (Tumor Necrosis Factor Alpha)
Key pro-inflammatory cytokine in immune activation. Available for human, mouse, rat, dog, and pig.
CRP (C-Reactive Protein)
Acute-phase marker for inflammation and infection models. Available for human, mouse, rat, and dog.
Antibody Pairs by Species
Select antibody pairs validated for your model organism:
Applications by Research Area
Inflammation & Immunity
| Target | Product | SKU | Research Focus |
|---|---|---|---|
| Granzyme B (GzmB) | Human GzmB Antibody Pair | STJAP00443 | Cytotoxic T-cell & NK cell function |
| CXCL2 (GRO Beta) | Human CXCL2 Antibody Pair | STJAP00494 | Neutrophil recruitment & inflammation |
| IFN-β (Interferon Beta) | Human IFN-β Antibody Pair | STJAP00032 | Antiviral immunity & autoimmune disease |
Oncology & Disease Biomarkers
| Target | Product | SKU | Research Focus |
|---|---|---|---|
| CA125 (Mucin-16) | Human CA125 Antibody Pair | STJAP00128 | Ovarian cancer marker & diagnostics |
| Aβ 1-40 (Amyloid Beta) | Human Aβ 1-40 Antibody Pair | STJAP00418 | Alzheimer's disease pathology |
| ADAM10 | Human ADAM10 Antibody Pair | STJAP00239 | APP processing & neurodegeneration |
Extracellular Matrix & Tissue Remodelling
| Target | Product | SKU | Research Focus |
|---|---|---|---|
| ADAMTS4 | Human ADAMTS4 Antibody Pair | STJAP00263 | Cartilage degradation & arthritis |
| ADAMTS5 | Human ADAMTS5 Antibody Pair | STJAP00475 | Connective tissue disorders |
| HTRA1 (Serine Protease) | Human HTRA1 Antibody Pair | STJAP00469 | ECM degradation & fibrosis |
Building Your Sandwich ELISA
Using Antibody Pairs Effectively
Our validated pairs simplify assay development, but optimisation of coating concentration, blocking conditions, and detection reagents may still improve performance for your specific samples:
1. Coating
Start with recommended capture antibody concentration. Optimise coating buffer pH and incubation time for maximum surface binding.
2. Blocking
Use protein-based blockers (BSA, casein) to minimise non-specific binding. Match blocking agent to your sample matrix.
3. Detection
Titrate detection antibody concentration. Ensure enzyme conjugate or secondary antibody compatibility with your readout system.
4. Validation
Run standard curves with recombinant protein. Verify linearity, sensitivity, and dynamic range across expected sample concentrations.
Need Custom Antibody Pair Development?
Our team can screen and validate antibody pairs for your specific target. Contact us to discuss custom assay development, conjugation services, or bulk orders.
Can't Find Your Target?
Our catalogue is constantly expanding. Let us know what you're looking for and we'll help source or develop the perfect antibody pair for your research.
Contact Our Antibody SpecialistsFor Research Use Only - All products are intended for research purposes and are not for use in diagnostic procedures