Human HTRA1 (Serine Protease Htra1) Antibody Pair (STJAP00469)
General Information
| Short Description | This HTRA1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rat/Rat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | HTRA1 |
| Gene ID | 5654 |
| Uniprot ID | HTRA1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human HTRA1 protien.Detection Immunogen: Recombinant Human HTRA1 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. |
| Protein Name | Serine Protease Htra1High-Temperature Requirement A Serine Peptidase 1L56Serine Protease 11 |
| Database Links | Reactome: R-HSA-1474228 |
| Cellular Localisation | Cell MembraneSecretedCytoplasmCytosolPredominantly SecretedAlso Found Associated With The Plasma Membrane |
| Alternative Antibody Pair Names | Serine Protease Htra1 antibody pairHigh-Temperature Requirement A Serine Peptidase 1 antibody pairL56 antibody pairSerine Protease 11 antibody pairHTRA1 antibody pairHTRA antibody pairPRSS11 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human HTRA1 (Serine Protease Htra1) Antibody Pair (STJAP00469)
This antibody pair detects human HTRA1 (Serine Protease HtrA1, UniProt Q92743) and is validated for reactivity against human samples only. Both the capture and detection antibodies were raised using recombinant human HTRA1 protein as the immunogen.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated, both at a concentration of 0.5 mg/mL. Both antibodies are rat-derived and purified by Protein A or G affinity chromatography.
This pair is validated for use in ELISA. It is intended for quantitative detection of human HTRA1 in serum, plasma, and other biological fluid sample types.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.