Human ADAMTS5 (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 5) Antibody Pair (STJAP00475)
General Information
| Short Description | This ADAMTS5 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | ADAMTS5 |
| Gene ID | 11096 |
| Uniprot ID | ATS5_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human ADAMTS5 protien.Detection Immunogen: Recombinant Human ADAMTS5 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | The precursor is cleaved by furin and PCSK7 outside of the cell. Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Can also be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion. |
| Function | Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN. Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration. Participates in development of brown adipose tissue and browning of white adipose tissue. Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection. |
| Protein Name | A Disintegrin And Metalloproteinase With Thrombospondin Motifs 5Adam-Ts 5Adam-Ts5Adamts-5A Disintegrin And Metalloproteinase With Thrombospondin Motifs 11Adam-Ts 11Adamts-11Admp-2Aggrecanase-2 |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-5083635Reactome: R-HSA-5173214 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Pair Names | A Disintegrin And Metalloproteinase With Thrombospondin Motifs 5 antibody pairAdam-Ts 5 antibody pairAdam-Ts5 antibody pairAdamts-5 antibody pairA Disintegrin And Metalloproteinase With Thrombospondin Motifs 11 antibody pairAdam-Ts 11 antibody pairAdamts-11 antibody pairAdmp-2 antibody pairAggrecanase-2 antibody pairADAMTS5 antibody pairADAMTS11 antibody pairADMP2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human ADAMTS5 (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 5) Antibody Pair (STJAP00475)
This antibody pair detects human ADAMTS5 (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 5), corresponding to UniProt entry Q9UNA0. Reactivity is confirmed for human samples only.
The capture antibody is goat-derived, purified by antigen affinity, and supplied unconjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is mouse-derived, purified by Protein A or G, biotinylated, and supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Validated sample types include serum, plasma, and other biological fluids. This product is intended for research use only and is not validated for diagnostic or therapeutic applications.
Both the capture and detection antibodies should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Both the capture and detection antibodies were raised against recombinant human ADAMTS5 protein. No specific immunogen region is defined in the product specifications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.