Human ADAMTS4 (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 4) Antibody Pair (STJAP00263)
General Information
| Short Description | This ADAMTS4 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | ADAMTS4 |
| Gene ID | 9507 |
| Uniprot ID | ATS4_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human ADAMTS4 protein.Detection Immunogen: Recombinant Human ADAMTS4 protein. |
Additional Info
| Post Translational Modifications | The precursor is cleaved by a furin endopeptidase. Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Can also be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion. |
| Function | Cleaves aggrecan, a cartilage proteoglycan, at the '392-Glu-|-Ala-393' site and may be involved in its turnover. Also cleaves COMP. May play an important role in the destruction of aggrecan in arthritic diseases. Could be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. |
| Protein Name | A Disintegrin And Metalloproteinase With Thrombospondin Motifs 4Adam-Ts 4Adam-Ts4Adamts-4Admp-1Aggrecanase-1 |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-5083635Reactome: R-HSA-5173214 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Pair Names | A Disintegrin And Metalloproteinase With Thrombospondin Motifs 4 antibody pairAdam-Ts 4 antibody pairAdam-Ts4 antibody pairAdamts-4 antibody pairAdmp-1 antibody pairAggrecanase-1 antibody pairADAMTS4 antibody pairKIAA0688 antibody pairUNQ769 antibody pairPRO1563 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human ADAMTS4 (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 4) Antibody Pair (STJAP00263)
This antibody pair is designed to detect human ADAMTS4 (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 4), corresponding to UniProt entry O75173. Reactivity has been confirmed for human samples only.
The capture antibody is an unconjugated goat polyclonal antibody, and the detection antibody is a biotin-conjugated mouse monoclonal antibody. Both components are supplied at 0.5 mg/mL.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product documentation.
Both the capture and detection antibodies were raised against recombinant human ADAMTS4 protein. No specific immunogen region is defined in the product details.
The capture antibody is purified by antigen affinity, while the detection antibody is purified using Protein A or G. Both components are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.