St John's Laboratory Ltd

Phospho-Smad3 antibody western blot Jurkat C6 CHO-K1 HeLa STJA0033914

Anti-Phospho-Smad3-Ser423/425 antibody [R09-6A2] (STJA0033914)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Hamster

£133.50 - £281.50

Choose Options

Smad3 antibody western blot 3T3 C6 STJA0034867

Anti-Smad3 antibody [R02-1I2] (STJA0034867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Hamster

£133.50 - £281.50

Choose Options

Immunofluorescence analysis of NIH/3T3 cells using Smad3 Rabbit polyclonal antibody (STJ11101192) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SMAD3 antibody (150-425) (STJ11101192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot analysis of lysates from C6 cells, using Phospho-Smad3-S423/S425 Rabbit mAb (STJ11102566) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-Smad3-S423/S425 antibody [S6MR] (STJ11102566)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-Smad3-T179 Rabbit pAb (STJ114841). HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SMAD3-T179 antibody (STJ114841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

Phospho-Smad3 antibody immunohistochemistry human skin tissue STJA0032933

Anti-Phospho-Smad3-Ser425 antibody [7H6-4E5-4H2] (STJA0032933)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

Choose Options

Smad3 antibody western blot 293T Jurkat rat mouse heart STJA0032967

Anti-Smad3 antibody [4H4-10A10-7B4] (STJA0032967)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

Choose Options

Immunofluorescence analysis of Hela cell. 1, Smad3 (phospho Ser204) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3A mouse monoclonal antibody (5G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-SMAD3-Ser204 antibody (170-219 aa) (STJ90523)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of Hela cell. 1, Smad3 (phospho Ser425) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). ATG5 mouse monoclonal antibody (3C7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-SMAD3-Ser425 antibody (376-425 aa) (STJ90745)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of various cells using Phospho-Smad3 (S208) Polyclonal Antibody

Anti-Phospho-SMAD3-Ser208 antibody (174-223 aa) (STJ90906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Immunofluorescence analysis of Hela cell. 1, Smad3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-actin monoclonal antibody (5B7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD3 antibody (145-194 aa) (STJ95699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Recombinant-SMAD3 antibody [RM2B46] (STJA0025119)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Rat

£300.50 - £457.50

Choose Options

Smad1 antibody western blot 293T cells STJA0036149

Anti-Smad1 antibody [R02-1J1] (STJA0036149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

Choose Options

Western blot analysis of 823 22RV1 HELA HEPG2 Cell Lysate, antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-PMEPA1 antibody (240-287 aa) (STJ98968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

Choose Options

Smad3 antibody immunocytochemistry A549 cells STJA0010795

Anti-SMAD3 antibody (202-219 aa) [R09-7H4] (STJA0010795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£133.50 - £281.50

Choose Options

Western blot analysis of various cells using Phospho-Smad1 (S465) Polyclonal Antibody

Anti-Phospho-SMAD1-Ser465 antibody (416-465 aa) (STJ90743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Anti-Smad1 antibody [SRM13316] (STJ11108046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

Choose Options

Anti-Smad3 antibody [SRM14491] (STJ11109221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

Choose Options

Human SMAD5 protein (Recombinant) (N-His) (STJP005905)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

Choose Options

STJ71682 (0. 5µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-LIP1/LKB1IP antibody (Internal) (STJ71682)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

Choose Options

Western blot analysis using Smad5 monoclonal antibody against mouse testis lysate.

Anti-SMAD5 antibody (C-Term) (STJ98549)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig/Sheep

£149.00 - £360.00

Choose Options

Smad5 antibody western blot C6 HeLa lysates STJA0011975

Anti-SMAD5 antibody (244-262 aa) [R07-2A5] (STJA0011975)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£133.50 - £281.50

Choose Options

SMAD5 C-terminus antibody western blot HeLa Jurkat K562 STJA0032369

Anti-Smad5 antibody [4B10-B10-B6] (STJA0032369)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human colon using Smad5 Rabbit polyclonal antibody (STJ25609) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Smad5 antibody (186-465) (STJ25609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Western blot detection of SMAD5 (C-terminus) in Hela, Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.

Anti-SMAD5 antibody (C-Term) [4B10-B10-B6] (STJ99081)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/FC

Reactivity :

Human

£149.00 - £360.00

Choose Options

Anti-Recombinant-SMAD5 antibody [RM2J88] (STJA0025854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Rat

£300.50 - £457.50

Choose Options

Phospho-Smad5 antibody western blot mouse rat brain STJA0035788

Anti-Phospho-Smad5-Ser463/465 antibody [R05-2U1] (STJA0035788)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

Choose Options

Western blot analysis of extracts from K562 cells, using Smad5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-SMAD5 antibody (Internal) (STJ96452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

Choose Options

Anti-Phospho-SMAD5-Ser463/465 antibody [PT0663R] (STJA0039233)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-Smad5-S463/S465 Rabbit mAb (STJ11102605) at 1:1000 dilution or Smad5 antibody (STJ25609). Hela cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-SMAD5-S463/S465 antibody [S5MR] (STJ11102605)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

Choose Options

Anti-VPS39 antibody (STJA0007128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

Choose Options

Anti-Smad5 antibody [PT0627R] (STJA0039290)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-Smad5-S463/S465 Rabbit polyclonal antibody (STJ11105818) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-SMAD5-S463/S465 antibody (STJ11105818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

Choose Options

STJ71512 (0. 1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Dachshund homolog 1 antibody (Internal) (STJ71512)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig

£312.50

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts of K-562 cells using 3 Mu g Smad5 antibody (STJ11101654). Western blot was performed from the immunoprecipitate using Smad5 antibody (STJ11101654) at a dilution of 1:1000.

Anti-SMAD5 antibody (200-300) [S4MR] (STJ11101654)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

Choose Options

Western blot analysis of lysates from wild type (WT) and Smad5 knockout (KO) 293T (KO) cells, using [KO Validated] Smad5 Rabbit monoclonal antibody (STJ11104940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-SMAD5 antibody (186-260) [S4940RM] (STJ11104940)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

Choose Options

Western blot analysis of C6, using Phospho-Smad5-S465 Rabbit monoclonal antibody (STJ11105872) at 1:2000 dilution. C6 cells were treated by MG132 (50 Mu M) and TGF Beta at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-SMAD5-S465 antibody [S5872RM] (STJ11105872)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

Choose Options

Western blot analysis of lysates from Rat brain, using VPS39 Rabbit polyclonal antibody (STJ115049) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-VPS39 antibody (606-875) (STJ115049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Human DACH1 protein (Recombinant) (N-His-SUMO) (STJP008999)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

Choose Options

Anti-DACH1 antibody (STJA0006737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

Choose Options

Mouse TGFBR1 protein (Recombinant-Active) (C-Fc) (STJP012847)

Host :

Mammalian Cells

Applications :

ELISA/WB

£575.50

Choose Options

Mouse TGFBR2/TGFR-2 protein (Recombinant) (N-His-SUMO) (STJP009867)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

Choose Options

Mouse TGFBR2/TGFR-2 protein (Recombinant) (C-Fc) (STJP013912)

Host :

Mammalian Cells

Applications :

ELISA/WB

£0.00

Add to Cart The item has been added

Mouse TGFBR2/TGFR-2 protein (Recombinant-Active) (C-hFc-His) (STJP012153)

Host :

Mammalian Cells

Applications :

ELISA/WB

£503.50

Choose Options

Human SMAD9 protein (Recombinant) (N-GST) (STJP003224)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

Choose Options

Western blot analysis of 293F, using DACH1 antibody (STJ28975) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DACH1 antibody (450-570) (STJ28975)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

Choose Options

Human PPM1A protein (Recombinant) (N-His) (STJP004631)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

Choose Options

Human PPM1A protein (Recombinant) (C-His) (STJP014489)

Host :

E.coli

Applications :

ELISA/WB

£503.50

Choose Options