St John's Laboratory Ltd

Anti-Phospho-Smad3-Ser423/425 antibody [PT0913R] (STJA0039804)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£150.00 - £540.00

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Phospho-Smad1 antibody western blot A549 U2OS C6 STJA0035097

Anti-Phospho-Smad1-Ser463/Ser465 antibody [R07-4A3] (STJA0035097)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of various cells using PMEPA1 Polyclonal Antibody diluted at 1：500

Anti-PMEPA1 antibody (101-150 aa) (STJ95165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Phospho-Smad3 antibody western blot PC-12 lysates STJA0035799

Anti-Phospho-Smad3-Ser423/425 antibody [R02-7Z7] (STJA0035799)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Western blot analysis of various cells using Smad1/5/9 Polyclonal Antibody diluted at 1:500 STJ95690

Anti-Smad1/5/9 antibody (10-59 aa) (STJ95690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 823 22RV1 HELA HEPG2 Cell Lysate, antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-PMEPA1 antibody (240-287 aa) (STJ98968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMAD1-Ser206 antibody (STJ196428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of HELA cells using Phospho-Smad3 (T179) Polyclonal Antibody

Anti-Phospho-SMAD3-Thr179 antibody (145-194 aa) (STJ90905)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human Liver Carcinoma Tissue using Smad3 Mouse mAb diluted at 1:200.

Anti-SMAD3 antibody (350-430 aa) [4C9] (STJ97772)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit polyclonal antibody (STJ11104508) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SMAD1 antibody (20-240) (STJ11104508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Recombinant-SMAD3 antibody [RM2B47] (STJA0025120)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/WB

Reactivity :

Human/Mouse/Rat/Hamster

£300.50 - £457.50

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Anti-Recombinant-Phospho-SMAD1-Ser463/Ser465 antibody [RM2G70] (STJA0025638)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/WB

Reactivity :

Human/Rat

£300.50 - £457.50

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Anti-Smad1 antibody [SRM14414] (STJ11109144)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunohistochemistry analysis of Smad3 in paraffin-embedded human tonsil using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD3 antibody (100-200) [S2MR] (STJ11101652)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of various lysates using (STJ11103777) at 1:1000 dilution. Hela cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-Smad3-S423/S425 antibody (STJ11103777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-Smad1 (S465) Polyclonal Antibody

Anti-Phospho-SMAD1-Ser465 antibody (416-465 aa) (STJ90743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Smad1 antibody [SRM13316] (STJ11108046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Anti-Smad3 antibody [SRM14491] (STJ11109221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Human SMAD5 protein (Recombinant) (N-His) (STJP005905)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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STJ71682 (0. 5µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-LIP1/LKB1IP antibody (Internal) (STJ71682)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-Smad5-S463/S465 Rabbit polyclonal antibody (STJ11105818) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-SMAD5-S463/S465 antibody (STJ11105818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Smad5-S463/S465 Rabbit mAb (STJ11102605) at 1:1000 dilution or Smad5 antibody (STJ25609). Hela cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-SMAD5-S463/S465 antibody [S5MR] (STJ11102605)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-VPS39 antibody (STJA0007128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Smad5 antibody [PT0627R] (STJA0039290)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of extracts from K562 cells, using Smad5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-SMAD5 antibody (Internal) (STJ96452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

£49.00 - £261.00

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Anti-Phospho-SMAD5-Ser463/465 antibody [PT0663R] (STJA0039233)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis using Smad5 monoclonal antibody against mouse testis lysate.

Anti-SMAD5 antibody (C-Term) (STJ98549)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC

Reactivity :

Human/Mouse/Rat/Cow/Chicken/Pig/Sheep

£149.00 - £360.00

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Smad5 antibody western blot C6 HeLa lysates STJA0011975

Anti-SMAD5 antibody (244-262 aa) [R07-2A5] (STJA0011975)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£133.50 - £281.50

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SMAD5 C-terminus antibody western blot HeLa Jurkat K562 STJA0032369

Anti-Smad5 antibody [4B10-B10-B6] (STJA0032369)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/FC

Reactivity :

Human

£133.50 - £281.50

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Immunohistochemistry analysis of paraffin-embedded human colon using Smad5 Rabbit polyclonal antibody (STJ25609) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Smad5 antibody (186-465) (STJ25609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot detection of SMAD5 (C-terminus) in Hela, Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.

Anti-SMAD5 antibody (C-Term) [4B10-B10-B6] (STJ99081)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/FC

Reactivity :

Human

£149.00 - £360.00

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Anti-Recombinant-SMAD5 antibody [RM2J88] (STJA0025854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Rat

£300.50 - £457.50

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Phospho-Smad5 antibody western blot mouse rat brain STJA0035788

Anti-Phospho-Smad5-Ser463/465 antibody [R05-2U1] (STJA0035788)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunoprecipitation analysis of 300 Mu g extracts of K-562 cells using 3 Mu g Smad5 antibody (STJ11101654). Western blot was performed from the immunoprecipitate using Smad5 antibody (STJ11101654) at a dilution of 1:1000.

Anti-SMAD5 antibody (200-300) [S4MR] (STJ11101654)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of lysates from wild type (WT) and Smad5 knockout (KO) 293T (KO) cells, using [KO Validated] Smad5 Rabbit monoclonal antibody (STJ11104940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-SMAD5 antibody (186-260) [S4940RM] (STJ11104940)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Western blot analysis of C6, using Phospho-Smad5-S465 Rabbit monoclonal antibody (STJ11105872) at 1:2000 dilution. C6 cells were treated by MG132 (50 Mu M) and TGF Beta at 37 °C for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-SMAD5-S465 antibody [S5872RM] (STJ11105872)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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STJ71512 (0. 1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Dachshund homolog 1 antibody (Internal) (STJ71512)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig

£312.50

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Western blot analysis of lysates from Rat brain, using VPS39 Rabbit polyclonal antibody (STJ115049) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-VPS39 antibody (606-875) (STJ115049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Human DACH1 protein (Recombinant) (N-His-SUMO) (STJP008999)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-DACH1 antibody (STJA0006737)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Mouse TGFBR2/TGFR-2 protein (Recombinant) (N-His-SUMO) (STJP009867)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Mouse TGFBR2/TGFR-2 protein (Recombinant) (C-Fc) (STJP013912)

Host :

Mammalian Cells

Applications :

ELISA/WB

£0.00

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Mouse TGFBR1 protein (Recombinant-Active) (C-Fc) (STJP012847)

Host :

Mammalian Cells

Applications :

ELISA/WB

£575.50

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Mouse TGFBR2/TGFR-2 protein (Recombinant-Active) (C-hFc-His) (STJP012153)

Host :

Mammalian Cells

Applications :

ELISA/WB

£503.50

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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMAD2-Ser255 antibody (STJA0009832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Recombinant-Phospho-SMAD2-S250 antibody [RM2G65] (STJA0025633)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Human SMAD2 protein (Recombinant) (N-His) (STJP005472)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Human PPM1A protein (Recombinant) (C-His) (STJP014489)

Host :

E.coli

Applications :

ELISA/WB

£503.50

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