• Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit polyclonal antibody (STJ11104508) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Smad1 Rabbit polyclonal antibody (STJ11104508) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using Smad1 Rabbit polyclonal antibody (STJ11104508) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using Smad1 Rabbit polyclonal antibody (STJ11104508) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from wild type (WT) and Smad1 knockout (KO) HeLa cells, using Smad1 antibody (STJ11104508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using Smad1 antibody (STJ11104508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SMAD1 antibody (20-240) (STJ11104508)

SKU:
STJ11104508

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mothers Against Decapentaplegic Homolog 1 (20-240) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMAD1
Gene ID: 4086
Uniprot ID: SMAD1_HUMAN
Immunogen Region: 20-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human Smad1 (NP_001003688.1).
Immunogen Sequence: WKQGDEEEKWAEKAVDALVK KLKKKKGAMEELEKALSCPG QPSNCVTIPRSLDGRLQVSH RKGLPHVIYCRVWRWPDLQS HHELKPLECCEFPFGSKQKE VCINPYHYKRVESPVLPPVL VPRHSEYNPQHSLLAQFRNL GQNEPHMPLNATFPDSFQQP NSHPFPHSPNSSYPNSPGSS SSTYPHSPTSSDPGSPFQMP ADTPPPAYLPPEDPMTQDGS Q
Tissue Specificity Ubiquitous. Highest expression seen in the heart and skeletal muscle.
Post Translational Modifications Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4. Phosphorylation by ERK2 MAP kinase in response to EGF or HGF prevents SMAD1 nuclear accumulation and transcriptional activity in response to BMP. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm. Dephosphorylation is inhibited by association with EGR1. Phosphorylation by CDK8/9 creates binding sites for YAP1, and subsequent phosphorylation by GSK3 switches off YAP1 binding and adds binding sites for SMURF1. Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm. Phospho-SMAD1 is ubiquitinated by CHIP leading to disruption of the SMAD1-SMAD4 complex.
Function Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import.
Protein Name Mothers Against Decapentaplegic Homolog 1
Mad Homolog 1
Mothers Against Dpp Homolog 1
Jv4-1
Mad-Related Protein 1
Smad Family Member 1
Smad 1
Smad1
Hsmad1
Transforming Growth Factor-Beta-Signaling Protein 1
Bsp-1
Database Links Reactome: R-HSA-201451
Reactome: R-HSA-5689880
Reactome: R-HSA-8941326
Reactome: R-HSA-9733709
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic In The Absence Of Ligand
Migrates To The Nucleus When Complexed With Smad4
Co-Localizes With Lemd3 At The Nucleus Inner Membrane
Exported From The Nucleus To The Cytoplasm When Dephosphorylated
Alternative Antibody Names Anti-Mothers Against Decapentaplegic Homolog 1 antibody
Anti-Mad Homolog 1 antibody
Anti-Mothers Against Dpp Homolog 1 antibody
Anti-Jv4-1 antibody
Anti-Mad-Related Protein 1 antibody
Anti-Smad Family Member 1 antibody
Anti-Smad 1 antibody
Anti-Smad1 antibody
Anti-Hsmad1 antibody
Anti-Transforming Growth Factor-Beta-Signaling Protein 1 antibody
Anti-Bsp-1 antibody
Anti-SMAD1 antibody
Anti-BSP1 antibody
Anti-MADH1 antibody
Anti-MADR1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance