• Immunocytochemistry of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.
  • Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution) , followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.
  • Western blot detection of SMAD5 (C-terminus) in Hela, Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.

Anti-SMAD5 antibody (C-Term) [4B10-B10-B6] (STJ99081)

SKU:
STJ99081

€173.75 - €420.00
Current Stock:
Host: Mouse
Applications: WB/ICC/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Mothers against decapentaplegic homolog 5 (C-Term) is suitable for use in Western Blot, Immunocytochemistry and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: 4B10-B10-B6
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
ICC 1:75 FCM 1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMAD5
Gene ID: 4090
Uniprot ID: SMAD5_HUMAN
Immunogen Region: C-Term
Specificity: This antibody detects endogenous levels of SMAD5 (C-terminus) and does not cross-react with related proteins.
Immunogen: Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.
Post Translational Modifications Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.
Function Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis.
Protein Name Mothers Against Decapentaplegic Homolog 5
Mad Homolog 5
Mothers Against Dpp Homolog 5
Jv5-1
Smad Family Member 5
Smad 5
Smad5
Hsmad5
Database Links Reactome: R-HSA-201451
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Cytoplasmic In The Absence Of Ligand
Migrates To The Nucleus When Complexed With Smad4
Alternative Antibody Names Anti-Mothers Against Decapentaplegic Homolog 5 antibody
Anti-Mad Homolog 5 antibody
Anti-Mothers Against Dpp Homolog 5 antibody
Anti-Jv5-1 antibody
Anti-Smad Family Member 5 antibody
Anti-Smad 5 antibody
Anti-Smad5 antibody
Anti-Hsmad5 antibody
Anti-SMAD5 antibody
Anti-MADH5 antibody

Information sourced from Uniprot.org

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