St John's Laboratory Ltd

ErbB 2 Blocking Peptide peptide (STJ505382)

Applications :

Immunodepletion/Immunocompetition

£260.50

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Anti-P70 S6 Kinase beta antibody (400-450) (STJ505929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-P70 S6 Kinase beta antibody (400-450) {FITC} (STJ505931)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-CD340/ERBB2 antibody (Met1-Thr652) [1A064] (STJA0021902)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£197.50 - £275.50

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Anti-CD340/ERBB2/HER2 antibody [FRP5] (STJA0022313)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/WB

Reactivity :

Human

£195.50 - £1,319.50

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Human CD340/ERBB2 protein (Recombinant) (N-His) (STJP003674)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Anti-HER2/ErbB2 antibody [SRM13217] (STJ11107947)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £554.50

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Human ErbB-2/HER2/CD340 protein (Recombinant) (C-His&Avi) {Biotin} (STJP019947)

Host :

HEK293 cells

£541.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T421/S424 pAb (STJ11101140) at 1:1000 dilution or P70 S6K antibody (STJ119250). NIH/3T3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-RPS6KB1-T421/S424 antibody (STJ11101140)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-S371 Rabbit pAb (STJ11101156) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RPS6KB1-S371 antibody (STJ11101156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Immunofluorescence analysis of NIH/3T3 cells using p70 S6 Kinase 1 Rabbit polyclonal antibody (STJ119077) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RPS6KB1 antibody (426-525) (STJ119077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of K562 using p-Neu (Y1112) antibody. Antibody was diluted at 1:500

Anti-Phospho-ERBB2-Tyr1112 antibody (1081-1130 aa) (STJ90573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-RPS6KA1-Thr573 antibody (539-588 aa) (STJ91047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-RPS6KA1 antibody (539-588 aa) (STJ95545)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ70247 (0. 3µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-p70S6K/RPS6KB1 antibody (C-Term) (STJ70247)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ73338 (4µg/ml) staining of paraffin embedded Human breast cancer (Her+ left, triple negative right). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-ERBB2/HER2 antibody (182-194) (STJ73338)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human

£312.50

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ErbB 2 Positive Control peptide (Phospho) (STJ504716)

Applications :

WB

£330.50

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Biotinylated Human Her2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Human Her2/ErbB2 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001767)

Host :

HEK293

£570.50 - £1,762.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Her-2 (ABT088) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7Predicted band size: 137kDaObserved band size: 200kDa

Anti-ERBB2 antibody (300-400 aa) [ABT008] (STJ197162)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-S371 Rabbit pAb (STJ11105784) at 1:400 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-RPS6KB1-S371 antibody (STJ11105784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£126.50 - £618.50

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Active Recombinant Human ErbB-2/HER2/CD340 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100-120 kDa.

Active Human ErbB-2/HER2/CD340 protein (Recombinant) (C-His) (STJP002595)

Host :

HEK293 cells

£88.50 - £266.50

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Human EGFR2 (Receptor Tyrosine-Protein Kinase Erbb-2) Antibody Pair (STJAP00428)

Host :

Rabbit/Rabbit

Applications :

ELISA

Reactivity :

Human

£484.50

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Anti-CD340/ERBB2/HER2 antibody [MAB 2C4] (STJA0027264)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£315.50

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Anti-CD340/ERBB2/HER2 antibody [MAB 2C4] {FITC} (STJA0028031)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£315.50

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Anti-CD340/ERBB2/HER2/NEU antibody [A21] {PE} (STJA0030930)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£391.50

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Anti-CD340/ERBB2/HER2 nanobody [SAA0836] (STJN000017)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/RIA

Reactivity :

Human

£195.50 - £1,319.50

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Human CD340/ERBB2/HER2/NEU protein (Recombinant) (C-Fc) (STJP009510)

Host :

Mammalian Cells

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£267.50 - £2,006.50

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S6K1 antibody western blot C6 muscle STJA0034837

Anti-S6K1 antibody [R03-2B2] (STJA0034837)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (STJ11101097) at 1:500 dilution. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-RPS6KB1-T389 antibody (STJ11101097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-RPS6KA1-Ser227 antibody (STJ196410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from K562 cells, NIH/3T3 cells, and COLO205 cells, using p70 S6 Kinase STJ90373

Anti-Phospho-RPS6KB1-Ser434 antibody (401-450 aa) (STJ90373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis of 293 cells using Phospho-Rsk-1 (S380) Polyclonal Antibody

Anti-Phospho-RPS6KA1-Ser380 antibody (346-395 aa) (STJ90474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using p90 RSK (Phospho-Thr359+Ser363) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6KA1-Thr359/S363 antibody (331-380 aa) (STJ90825)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, p70 S6 kinase Alpha (phospho Thr412) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-RPS6KB1-Thr412 antibody (355-450 aa) (STJ91045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HT-29 serum 20% 15' using p-p70 S6 kinase Alpha (S418) antibody. Antibody was diluted at 1:1000

Anti-Phospho-RPS6KB1-Ser418 antibody (384-433 aa) (STJ91046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of HUVEC cells, using HER2 (Phospho-Thr686) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ERBB2-Thr686 antibody (661-710 aa) (STJ91335)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, p70 S6 kinase Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CK7 monoclonal antibody (12D7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-RPS6KB1 antibody (337-386 aa) (STJ94920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using P70 S6 Kinase Mouse mAb diluted at 1:200.

Anti-RPS6KB1 antibody [1C7] (STJ97708)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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ErbB 2 Blocking Peptide peptide (STJ504218)

Applications :

Immunodepletion/Immunocompetition

£260.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Her-2 (ABT338) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7Predicted band size: 137kDaObserved band size: 200kDa

Anti-ERBB2 antibody (300-400 aa) [ABT338] (STJ197087)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-Recombinant-S6K1/RPS6KB1 antibody [RM1S36] (STJA0024218)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Phospho-RPS6KA1/RSK1 p90-Ser380 antibody [RM2G36] (STJA0025604)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/WB

Reactivity :

Mouse/Hamster

£300.50 - £457.50

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Anti-CD340/ERBB2/HER2 antibody [4D5V8] {PE} (STJA0030928)

Clonality :

Monoclonal

Host :

Human

Applications :

FC

Reactivity :

Human

£228.50

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Anti-CD340/ERBB2/HER2/NEU nanobody [SAA1045] (STJN000097)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£195.50 - £1,319.50

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Anti-CD340/ERBB2/HER2 nanobody [11A4] (STJN000571)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£195.50 - £1,319.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T389 Rabbit pAb (STJ113500) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-RPS6KB1-T389 antibody (STJ113500)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of U-2 OS cells using p70 S6 Kinase 1 1 Rabbit polyclonal antibody (STJ25412) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RPS6KB1 antibody (400-525) (STJ25412)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-RPS6KB1-Thr389 antibody (STJ196372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Cow

£49.00 - £261.00

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