St John's Laboratory Ltd

STJ71529 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ARK5/NUAK1 antibody (Internal) (STJ71529)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Rat/Cow

£312.50

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Western blot phosphorylated DGCR8 protein 120 kDa in mouse nuclei lysate STJA0003611

Anti-Phospho-DGCR8-Ser377 antibody (STJA0003611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£488.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g DGCR8 antibody (STJ11100564). Western blot was performed from the immunoprecipitate using DGCR8 antibody (STJ11100564) at a dilution of 1:1000.

Anti-DGCR8 antibody (180-340) (STJ11100564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using ERdj3 Polyclonal Antibody diluted at 1：2000

Anti-DNAJB11 antibody (31-80 aa) (STJ92981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NUAK1 antibody (530-610 aa) (STJ190113)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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DGCR8 antibody immunocytochemistry K562 cells STJA0011234

Anti-DGCR8 antibody (424-773 aa) [R05-5A6] (STJA0011234)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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TREX1 antibody immunocytochemistry HeLa cells STJA0033838

Anti-TREX1 antibody [R05-1D6] (STJA0033838)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of extracts of various cell lines, using TREX1 Rabbit monoclonal antibody (STJ11101956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TREX1 antibody (150-314) [S6MR] (STJ11101956)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunofluorescence analysis of PC-12 cells using TREX1 Rabbit polyclonal antibody (STJ28861) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-TREX1 antibody (1-369) (STJ28861)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TREX1 antibody (220-245) {FITC} (STJ503389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IF/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-PLEKHO1 antibody (STJA0007418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of C6 cells using SKP2 Rabbit polyclonal antibody (STJ116316) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SKP2 antibody (1-100) (STJ116316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from rat heart, using NUAK1 Rabbit polyclonal antibody (STJ119461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NUAK1 antibody (362-661) (STJ119461)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TAOK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-TAOK1 antibody (431-480 aa) (STJ95247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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STJ70109 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-SIAH1 antibody (N-Term) (STJ70109)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-SKP2 antibody [SKP2-8D9] (STJ16100869)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/ELISA/IP

Reactivity :

Human/Non-Human Primates

£542.50

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Human SKP2 (S-Phase Kinase Associated Protein 2) Sandwich ELISA Kit Ready-To-Use Kit (STJE0018570)

Applications :

ELISA

Reactivity :

Human

£440.50 - £614.50

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Anti-TIF1/TRIM24 IP antibody [SAA0265] (STJA0018780)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£195.50 - £1,319.50

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Anti-Recombinant-Phospho-TAOK1/2/3-S181 + S181 + S177 antibody [RM2M45] (STJA0026108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Phospho-TAOK1/2/3-Ser181/Ser181/Ser177 antibody [RM2M46] (STJA0026109)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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SDS-PAGE of recombinant human TIGAR C12orf5 protein under reducing conditions

Human TIGAR/C12orf5 protein (Recombinant) (His-Tag) (STJP016973)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £1,191.50

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SDS-PAGE gel of recombinant human SIAH1 protein 3µg under reducing conditions Coomassie blue stain

Human SIAH1 protein (Recombinant) (His-Tag) (STJP017778)

Host :

E.coli

Applications :

SDS-PAGE

£158.50 - £965.50

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Western blot analysis of various cells using TIF1 Alpha Polyclonal Antibody diluted at 1：1000

Anti-TRIM24 antibody (1001-1050 aa) (STJ96019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Mouse BRCA2 (Breast Cancer Susceptibility Protein 2) Sandwich ELISA Kit (STJE0007547)

Applications :

ELISA

Reactivity :

Mouse

£413.50 - £575.50

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Western Blot analysis of mouse brainrat-brain, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NUAK1-Ser600 antibody (STJA0009944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Recombinant-TRIM24 antibody [RM1D62] (STJA0022957)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human

£300.50 - £457.50

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Anti-TRIM24 antibody [4G6C2] (STJ16103602)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF

Reactivity :

Human

£746.50

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SKP2 antibody western blot Jurkat NIH/3T3 STJA0036580

Anti-SKP2 antibody [R02-6V3] (STJA0036580)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of L929 cells using SIAH1 Rabbit polyclonal antibody (STJ25527) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SIAH1 antibody (1-313) (STJ25527)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ70306 (4µg/ml) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-TIF1A/TRIM24 antibody (C-Term) (STJ70306)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Cow

£312.50

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Immunofluorescence analysis of U2OS cells using TIGAR Rabbit polyclonal antibody (STJ11103300) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-TIGAR antibody (100-200) (STJ11103300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PLEKHO1 antibody (STJA0004461)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£139.50 - £403.50

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Anti-Recombinant-TAOK1 antibody [RM2M47] (STJA0026110)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Human TAOK1 protein (Recombinant) (N-His) (STJP007858)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Human TIGAR protein (Recombinant) (N-His) (STJP009477)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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SKP2 antibody immunocytochemistry HeLa cells STJA0035362

Anti-SKP2 antibody [R08-0A2] (STJA0035362)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from HUVEC cells treated with UV 15', using Chk1 (Phospho-Ser296) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser296 antibody (266-315 aa) (STJ90849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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STJ71518 (2µg/ml) staining of Rat Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CHEK1 antibody (Internal) (STJ71518)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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Western blot analysis of various lysates, using Chk1 Rabbit polyclonal antibody (STJ11104296) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: Q60s.

Anti-CHEK1 antibody (148-307) (STJ11104296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of extracts of K-562 cells, using Chk1 antibody (STJ11104686) at1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-CHEK1 antibody (250-350) [S4686RM] (STJ11104686)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Western blot analysis of 293T, using Phospho-Chk1 (S280) Rabbit monoclonal antibody (STJ11105881) at 1:500 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-CHEK1-S280 antibody [S5881RM] (STJ11105881)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Chk1 antibody [PT0606R] (STJA0039379)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Phospho-Histone H2AX Ser139 antibody western blot 3T3 STJA0033231

Anti-Phospho-Histone H2A.X-Ser139 antibody [7G9-H4] (STJA0033231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£133.50 - £281.50

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Phospho-Histone H2AX antibody western blot Jurkat STJA0036230

Anti-Phospho-Histone H2A.X-Ser139 antibody [R03-2Z1] (STJA0036230)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Chk1 antibody western blot THP-1 3T3 PC-12 STJA0035707

Anti-Chk1 antibody [R02-1D6] (STJA0035707)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S296 Rabbit mAb (STJ11102618) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes and Doxorubicin (0. 5 uM) at 37 °C for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-CHEK1-S296 antibody [S8MR] (STJ11102618)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of lysates from MCF7 cells, using Phospho-Chk1-S317 Rabbit pAb (STJ22072). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CHEK1-S317 antibody (STJ22072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Chk1-S345 Rabbit pAb (STJ29344) at 1:500 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-CHEK1-S345 antibody (STJ29344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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