Anti-TIGAR antibody (100-200) (STJ11103300)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TIGAR (100-200) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TIGAR |
| Gene ID | 57103 |
| Uniprot ID | TIGAR_HUMAN |
| Immunogen Region | 100-200 |
| Immunogen Sequence | LSELRAMAKAAREECPVFTP PGGETLDQVKMRGIDFFEFL CQLILKEADQKEQFSQGSPS NCLETSLAEIFPLGKNHSSK VNSDSGIPGLAASVLVVSHG A |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TIGAR (NP_065108.1). |
Additional Info
| Tissue Specificity | Expressed in the brain. Expressed in breast tumors. Expressed in glioblastomas. |
| Function | Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate. Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production. Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death. Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity. In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage. Plays a role in adult intestinal regeneration.contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation. Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions. Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner. Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses. Involved in intestinal tumor progression. |
| Protein Name | Fructose-2 -6-Bisphosphatase TigarTp53-Induced Glycolysis And Apoptosis RegulatorTp53-Induced Glycolysis Regulatory Phosphatase |
| Database Links | Reactome: R-HSA-5628897 |
| Cellular Localisation | CytoplasmNucleusMitochondrionTranslocated To The Mitochondria During Hypoxia In A Hif1a-Dependent MannerColocalizes With Hk2 In The Mitochondria During HypoxiaTranslocated To The Nucleus During Hypoxia And/Or Genome Stress-Induced Dna Damage Responses In Cancer CellsTranslocation To The Mitochondria Is Enhanced In Ischemic Cortex After Reperfusion And/Or During Oxygen And Glucose Deprivation (Ogd)/Reoxygenation Insult In Primary Neurons |
| Alternative Antibody Names | Anti-Fructose-2 -6-Bisphosphatase Tigar antibodyAnti-Tp53-Induced Glycolysis And Apoptosis Regulator antibodyAnti-Tp53-Induced Glycolysis Regulatory Phosphatase antibodyAnti-TIGAR antibodyAnti-C12orf5 antibody |
Information sourced from Uniprot.org