Anti-SKP2 antibody (1-100) (STJ116316)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SKP2 (1-100) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SKP2 |
| Gene ID | 6502 |
| Uniprot ID | SKP2_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MHRKHLQEIPDLSSNVATSF TWGWDSSKTSELLSGMGVSA LEKEEPDSENIPQELLSNLG HPESPPRKRLKSKGSDKDFV IVRRPKLNRENFPGVSWDSL |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SKP2 (NP_005974.2). |
Additional Info
| Post Translational Modifications | Phosphorylated on serine and threonine resudues in response to DNA damage, promoting 'Lys-63'-linked ubiquitination of NBN. Ubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Deubiquitinated by USP13. Acetylation at Lys-68 and Lys-71 increases stability through impairment of APC/C-mediated proteolysis and promotes cytoplasmic retention. Deacetylated by SIRT3. |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration. Following phosphorylation in response to DNA damage, mediates 'Lys-63'-linked ubiquitination of NBN, promoting ATM recruitment to DNA damage sites and DNA repair via homologous recombination. Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus. |
| Protein Name | S-Phase Kinase-Associated Protein 2Cyclin-A/Cdk2-Associated Protein P45F-Box Protein Skp2F-Box/Lrr-Repeat Protein 1P45skp2 |
| Database Links | Reactome: R-HSA-174178Reactome: R-HSA-187577Reactome: R-HSA-5689880Reactome: R-HSA-68949Reactome: R-HSA-69231Reactome: R-HSA-8939902Reactome: R-HSA-8951664Reactome: R-HSA-9687136Reactome: R-HSA-9708530Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-S-Phase Kinase-Associated Protein 2 antibodyAnti-Cyclin-A/Cdk2-Associated Protein P45 antibodyAnti-F-Box Protein Skp2 antibodyAnti-F-Box/Lrr-Repeat Protein 1 antibodyAnti-P45skp2 antibodyAnti-SKP2 antibodyAnti-FBXL1 antibody |
Information sourced from Uniprot.org