Vesicle-Mediated Transport | Research Area | St John's Laboratory

Western blot analysis of extracts of HeLa cells, using AP2M1 antibody (STJ115897) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AP2M1 antibody (134-433) (STJ115897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using AP2M1 Rabbit polyclonal antibody (STJ22628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-AP2M1 antibody (134-433) (STJ22628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunofluorescence analysis of U-251MG cells using AP2M1 Rabbit monoclonal antibody (STJ11101275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AP2M1 antibody (300-400) [S5MR] (STJ11101275)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from Mouse brain, using AP2S1 Rabbit mAb (STJ11103654) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-AP2S1 antibody (1-100) [S4MR] (STJ11103654)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$163.53 - $719.67

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Western blot analysis of various lysates using AP2S1 Rabbit polyclonal antibody (STJ114160) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-AP2S1 antibody (1-142) (STJ114160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AP2S1 antibody (26-76 aa) (STJ194906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-AP3B1 antibody (525-575) (STJA0006666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of various cell lines, using AP3B1 antibody (STJ29099) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AP3B1 antibody (895-1094) (STJ29099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AP4B1 antibody (595-645 aa) (STJ195839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AP4E1 antibody (104-154 aa) (STJ195182)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-AP4M1 antibody (312-362 aa) (STJ198137)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-AP4S1 antibody (69-119 aa) (STJ198164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded mouse brain using APOA1 antibody (STJ116143) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-APOA1 antibody (25-267) (STJ116143)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of HepG2 cells using APOA1 Rabbit polyclonal antibody (STJ22644) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-APOA1 antibody (25-267) (STJ22644)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of K562 cells using ApoA-I Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-APOA1 antibody (81-130 aa) (STJ96804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-APOA1 antibody [2001] (STJ400012)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$628.59 - $1,337.91

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Anti-ApoA1 antibody [2002] (STJ400201)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$616.17 - $928.05

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Western blot analysis using ApoA-I monoclonal antibody against HepG2 cell lysate (1) and human serum (2).

Anti-APOA1 antibody [5F4F5] (STJ97832)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$205.62 - $496.80

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Western blot analysis of lysates from Mouse plasma using APOB Rabbit pAb (STJ11107041) at1:1000 dilution. <br>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection:ECL Basic Kit <br>Exposuretime:5s.

Anti-APOB antibody (1890-2190 aa) (STJ11107041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of mouse liver cells using ApoB Rabbit polyclonal antibody (STJ22648) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-APOB antibody (28-463) (STJ22648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using ApoB Rabbit monoclonal antibody (STJ11102012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-APOB antibody (50-150) [S2MR] (STJ11102012)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$163.53 - $719.67

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Anti-APOB antibody (STJA0010126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-APOB antibody [2101] (STJ400014)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$628.59 - $1,201.29

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Anti-APOB antibody [SAA1553] (STJA0019981)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$471.27 - $1,997.55

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Anti-APOB antibody [SRM13551] (STJ11108281)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using Apoe Rabbit polyclonal antibody (STJ118772) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Apoe antibody (211-311) (STJ118772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of 293T cells using ApoE Polyclonal Antibody diluted at 1：500

Anti-APOE antibody (37-86 aa) (STJ91638)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of HepG2 cells using APOE Rabbit polyclonal antibody (STJ29789) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ApoE antibody (41-307) (STJ29789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-APOE antibody (Internal) (STJ91639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-APOE antibody [1D7] (STJA0021669)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA

Reactivity :

Human

$471.27 - $1,997.55

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Western blot analysis using ApoE monoclonal antibody against HEK293 (1) and ApoE (AA: 20-267) -hIgGFc transfected HEK293 (2) cell lysate.

Anti-APOE antibody [1H4] (STJ97839)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$205.62 - $496.80

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Anti-ApoE antibody [PT0948R] (STJA0039789)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-APOE antibody [SAA0799] (STJA0019266)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FuncS/IHC/IP

Reactivity :

Human

$471.27 - $1,997.55

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Anti-APOE antibody [SRM14963] (STJ11109693)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Anti-APOE nanobody [SAA1224] (STJN000086)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/SPR

Reactivity :

Human

$522.33 - $2,252.85

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Immunohistochemistry analysis of paraffin-embedded Rat brain using APOL1 Rabbit pAb (STJ110252) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.

Anti-APOL1 antibody (179-398) (STJ110252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Rat

$152.49 - $697.59

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Western blot analysis of various cells using ApoL1 Polyclonal Antibody diluted at 1：1000

Anti-APOL1 antibody (261-310 aa) (STJ91642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Confocal imaging of Hep G2 cells using APOL1 Rabbit monoclonal antibody (STJ11102075, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-APOL1 antibody (303-398) [S5MR] (STJ11102075)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot analysis using ApoL1 monoclonal antibody against human plasma (1).

Anti-APOL1 antibody [1D4] (STJ97840)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$205.62 - $496.80

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Anti-APOL1 NTD antibody [3B6] (STJA0019661)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$522.33 - $2,252.85

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Anti-APOL1 NTD antibody [7D6] (STJA0019662)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

$522.33 - $2,252.85

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Anti-Apolipoprotein A1 antibody [SRM13608] (STJ11108338)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$0.00

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Anti-Apolipoprotein A1/APOA1 antibody [SRM13387] (STJ11108117)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$0.00

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Anti-Apolipoprotein A1/APOA1 antibody [SRM14738] (STJ11109468)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Immunofluorescence analysis of HeLa cells using APP antibody (STJ112892) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-APP antibody (1-100) (STJ112892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of U-87MG cells, using APP antibody (STJ118700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-APP antibody (1-100) (STJ118700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-amyloid-Alpha-beta (PTR2007) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: SH-SY5YPredicted band size: 86kDaObserved band size: 95kDa

Anti-APP antibody (200-300) [PTR2007] (STJA0006225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, Amyloid-Beta Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-APP antibody (221-270 aa) (STJ98585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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