Signal Transduction | Research Area | St John's Laboratory

Immunofluorescence analysis of A431 cells using HEY1 antibody (STJ118550) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-HEY1 antibody (114-129) (STJ118550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HEY1 antibody (STJ193066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using HEY2 antibody (STJ117337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HEY2 antibody (17-32) (STJ117337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, using HEY2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-HEY2 antibody (21-70 aa) (STJ93595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using HEYL Rabbit polyclonal antibody (STJ119728) at 1:2500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HEYL antibody (1-100) (STJ119728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-HEYL antibody (STJA0009272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HepG2 cells using HGF Rabbit polyclonal antibody (STJ23940) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HGF antibody (106-728) (STJ23940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of SH-SY5Y lysis using HGF antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-HGF antibody (500-600 aa) (STJ97652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using HGFAC antibody (STJ112153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HGFAC antibody (408-655) (STJ112153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from K562, Jurkat cells, using HGFA Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HGFAC antibody (C-Term) (STJ96435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Hrs Polyclonal Antibody diluted at 1：500

Anti-HGS antibody (301-350 aa) (STJ93593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using HGS antibody (STJ28094) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HGS antibody (548-777) (STJ28094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HGS antibody (C-Term) (STJ13101216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat Mouse

£331.50

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Western blot analysis of various lysates using HHAT Rabbit polyclonal antibody (STJ117365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HHAT antibody (1-100) (STJ117365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using HHIP Rabbit pAb (STJ117801) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-HHIP antibody (400-700) (STJ117801)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry of mouse lung with Anti-HIF-1-Alpha isoform 1 + 2 primary antibody (STJ501335) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-HIF-1-Alpha isoform 1 + 2 antibody (150-250) (STJ501335)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-HIF-1-Alpha isoform 1 + 2 antibody (150-250) {Biotin} (STJ501338)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-HIF-1-Alpha isoform 1 + 2 antibody (150-250) {FITC} (STJ501339)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemistry of mouse lung with Anti-HIF-1-Alpha isoform 1 primary antibody (STJ501334) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-HIF-1-Alpha isoform 1 antibody (790-826) (STJ501334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-HIF-1-Alpha isoform 1 antibody (790-826) {Biotin} (STJ501336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-HIF-1-Alpha isoform 1 antibody (790-826) {FITC} (STJ501337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-HIF1 Alpha antibody (552-826 aa) (STJ11106258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 1) Hela, 2) 293, 3)mouse braincells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-HIF1A antibody (328-377 aa) (STJ93498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-HIF1a antibody at 2500 dilution; HT1080 cells in the presence of CoCl2; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-HIF1A antibody (470 to 590aa) (STJ140124)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Western blot analysis of lysates from HepG2 cells, using HIF1 Alpha Rabbit polyclonal antibody (STJ29997) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HIF1A antibody (600-826) (STJ29997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from HeLa cells, using HIF1 Alpha Rabbit pAb (STJ113863) at 1:1000 dilution. HeLa cells were treated by Cobalt chloride (0. 1 mM) at 37 °C for 4 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-HIF1A antibody (670-826) (STJ113863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HIF1A antibody (780aa C-Term) (STJ140040)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Western blot analysis using HIF-1 Alpha monoclonal antibody against Cos7 (1) , HeLa (2) , Jurkat (3) , RAJI (4) and NIH/3T3 (5) cell lysate.

Anti-HIF1A antibody [1A3] (STJ98135)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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Western blot analysis of 1) HepG2 Cell, 2) HepG2 Treated with CoCl2 cell lysate using HIF-1α mouse Monoclonal monoclonal antibody diluted at 1:2,000.

Anti-HIF1A antibody [Mix] (STJ196669)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-HINT2 antibody (STJ11106998)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-HINT2 antibody (STJA0004739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-HINT2 antibody (STJA0004740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-HINT2 antibody (STJA0004741)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

£140.50 - £415.50

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Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (STJ29383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H2A antibody (1-143) (STJ29383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Western blot analysis of various lysates using Histone H2AX Rabbit pAb (STJ113097) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Histone H2A antibody (50-143) (STJ113097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Anti-Histone H2AX antibody (1-143 aa) (STJ11106040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Histone H3. 3 rabbit monoclonal antibody (STJ11102163). The ChIP sequencing results indicate the enrichment pattern of Histone H3. 3 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Histone H3 antibody (57-136) [S3MR] (STJ11102163)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Anti-Histone H3 antibody [SM0412] (STJA0010412)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-Histone H3.2 antibody (81-131 aa) (STJ194163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using [KO Validated] Histone H4 Rabbit pAb (STJ11106815) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Histone H4 antibody (1-100 aa) (STJ11106815)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using Histone H4 Rabbit polyclonal antibody (STJ119273) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Histone H4 antibody (1-100) (STJ119273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Western blot analysis of various lysates, using Histone H4 Rabbit polyclonal antibody (STJ29382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Histone H4 antibody (1-100) (STJ29382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Immunohistochemistry analysis of Histone H4 in paraffin-embedded mouse kidney using Histone H4 Rabbit monoclonal antibody (STJ11103155) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Histone H4 antibody (24-103) [S5MR] (STJ11103155)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Immunocytochemistry analysis of Histone H4 (green) in Hela using Histone H4 antibody, and DAPI (blue).

Anti-Histone H4 antibody (51-70 aa) [R05-8H9] (STJA0011428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/ChIP

Reactivity :

Human

£246.50

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Immunofluorescence analysis of human-liver tissue. 1, Histone H4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Histone H4 antibody (6-55 aa) (STJ93539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of various lysates using HMOX2 Rabbit polyclonal antibody (STJ24054) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMOX2 antibody (1-270) (STJ24054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-HMOX2 antibody (STJ192960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and hnRNP A1 knockout (KO) 293T cells, using [KO Validated] hnRNP A1 Rabbit polyclonal antibody (STJ11100067) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HNRNPA1 antibody (1-140) (STJ11100067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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