• Immunohistochemistry analysis of paraffin-embedded mouse spleen using CXCR1 antibody (STJ118814) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using CXCR1 antibody (STJ118814) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using CXCR1 antibody (STJ118814) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CXCR1 antibody (STJ118814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CXCR1 antibody (325-339) (STJ118814)

SKU:
STJ118814

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CXCR1 (325-339) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CXCR1
Gene ID: 3577
Uniprot ID: CXCR1_HUMAN
Immunogen Region: 325-339
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 325-339 of human CXCR1 (NP_000625.1).
Immunogen Sequence: LVSKEFLARHRVTSY
Function Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system.
Protein Name C-X-C Chemokine Receptor Type 1
Cxc-R1
Cxcr-1
Cdw128a
High Affinity Interleukin-8 Receptor A
Il-8r A
Il-8 Receptor Type 1
Cd Antigen Cd181
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-C-X-C Chemokine Receptor Type 1 antibody
Anti-Cxc-R1 antibody
Anti-Cxcr-1 antibody
Anti-Cdw128a antibody
Anti-High Affinity Interleukin-8 Receptor A antibody
Anti-Il-8r A antibody
Anti-Il-8 Receptor Type 1 antibody
Anti-Cd Antigen Cd181 antibody
Anti-CXCR1 antibody
Anti-CMKAR1 antibody
Anti-IL8RA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance