Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of various cells using Phospho-Akt2 (S474) Polyclonal Antibody diluted at 1：500

Anti-Phospho-AKT2-Ser474 antibody (432-481 aa) (STJ90169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ALK-Tyr1078 antibody (STJ196236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using ALK (Phospho-Tyr1096) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ALK-Tyr1096 antibody (1062-1111 aa) (STJ91196)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Phospho-ALK-Tyr1278 antibody (STJ196237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-ALK-Tyr1278/1282/1283 antibody (STJ196238)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from COS7 cells treated with anisomycin 25ug/ml 30', using ALK (Phospho-Tyr1507) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ALK-Tyr1507 antibody (1473-1522 aa) (STJ90845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£49.00 - £261.00

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Anti-Phospho-ALK-Tyr1586 antibody (STJ193212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ALK-Tyr1604 antibody (1570-1619 aa) (STJ91058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from HL-60 and HL-60 cells, using Phospho-ALK-Y1096 Rabbit pAb (STJ110843) at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight. HL-60 cells were treated by UV for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-ALK-Y1096 antibody (STJ110843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-Phospho-Alpha 1-Catenin-Tyr-148 antibody (STJA0041162)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat

£542.50

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Anti-Phospho-Alpha 6-Tubulin-Ser-165 antibody (STJA0041427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Anti-Phospho-alpha Tubulin-Tyr272 antibody [R06-9Z1] (STJA0035837)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-AMPK alpha 1-Ser496 antibody [R01-3B7] (STJA0035051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-Phospho-AMPK alpha 2-Ser345 antibody [R04-5T2] (STJA0036159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-AMPK alpha 2-Ser491 antibody [R05-9Q9] (STJA0036166)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-AMPK alpha 2-Thr172 antibody [R03-6K2] (STJA0035991)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Phospho-Amyloid Precursor Protein-Thr743 antibody [R07-4D0] (STJA0035052)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-Phospho-Androgen Receptor antibody (550-600) (STJ506200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-Phospho-Androgen Receptor antibody (550-600) {Biotin} (STJ506201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Anti-Phospho-Androgen Receptor antibody (550-600) {FITC} (STJ506202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£551.50

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Western blot analysis of extracts from NIH-3T3 cells, using Phospho-Annexin I (Y21) Polyclonal Antibody.

Anti-Phospho-ANXA1-Tyr21 antibody (STJ91361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from 293T and NIH/3T3 cells, using Phospho-AP2M1-T156 Rabbit pAb (STJ117921) at 1:1000 dilution. 293T cells were treated by Calyculin A for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Hydrogen Peroxide (2nM) for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-AP2M1-T156 antibody (STJ117921)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-AP2M1-Thr156 antibody (STJ194700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Phospho-AP2M1-Thr156 antibody [R03-8C7] (STJA0034025)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of lysates from HUVEC cells treated with PMA 125ng/ml 30', using APC (Phospho-Ser2054) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-APC-Ser2054 antibody (2020-2069 aa) (STJ90884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Mouse brain, using Phospho-APP-T668 Rabbit pAb (STJ22017) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-APP-T668 antibody (STJ22017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-APP-Thr668 antibody (STJ196240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Amyloid-Beta (T743) Polyclonal Antibody

Anti-Phospho-APP-Thr743 antibody (711-760 aa) (STJ91015)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma, using Androgen Receptor (Phospho-Ser213) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AR-Ser213 antibody (186-235 aa) (STJ90179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western Blot analysis of 1 HeLa cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-AR-Ser308 antibody (STJA0010044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma, using Androgen Receptor (Phospho-Ser650) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AR-Ser650 antibody (621-670 aa) (STJ90180)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-AR-Ser81 antibody (STJA0010072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells, using Androgen Receptor (Phospho-Ser94) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AR-Ser94 antibody (66-115 aa) (STJ91328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot of HeLa lysate showing specific immunolabeling of the ~110 kDa AR protein phosphorylated at Ser94 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-AR-Ser94 antibody (STJA0003579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

£488.50

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Western Blot analysis of 1 A549 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-AR-Tyr267 antibody (STJA0010079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from Jurkat cells treated with UV 15', using Androgen Receptor (Phospho-Tyr363) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-AR-Tyr363 antibody (331-380 aa) (STJ90599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western Blot analysis of HeLa cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-AR-Tyr534 antibody (STJA0010084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-ARAF-Ser299 antibody (266-315 aa) (STJ91197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ARAF-Tyr302 antibody (276-325 aa) (STJ91064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-Argonaute 2-Ser-387 antibody (STJA0041078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Anti-Phospho-Argonaute 2-Tyr-393 antibody (STJA0041079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£542.50

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Western blot analysis of 293T cells using Phospho-GRF-1 (Y1087) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ARHGAP35-Tyr1087 antibody (STJ90281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 3T3 cells using Phospho-GRF-1 (Y1105) Polyclonal Antibody

Anti-Phospho-ARHGAP35-Tyr1105 antibody (1071-1120 aa) (STJ90795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-ARHGAP42-Tyr376 antibody (STJ196241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using ARHGDIA (Phospho-Ser174) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ARHGDIA-Ser174 antibody (140-189 aa) (STJ90922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from C6 cells, using Phospho-ARHGEF2-S886 Rabbit pAb (STJ11101060) at 1:1000 dilution. C6 were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-ARHGEF2-S886 antibody (STJ11101060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Phospho-ARHGEF2-Ser885 antibody (851-900 aa) (STJ90558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Phospho-ARHGEF3 antibody (350-410) (STJ506109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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