Signal Transduction | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, TRIS-EDTA of pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-ANXA1 antibody (250-346 aa) [PT1761] (STJ196943)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

$0.00

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Western blot detection of Annexin A1 in A549, CHO-K1, COS7, C2C12, C6, 3T3 and Hela cell lysates using Annexin A1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.

Anti-ANXA1 antibody [5E4-D8-F12] (STJ99256)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

$205.62 - $496.80

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Anti-ANXA1 antibody [IHC512] (STJA0042008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

$181.47 - $483.69

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Anti-ANXA1 antibody [M021] (STJA0005256)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

$540.27

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Anti-ANXA1/Annexin A1 antibody [SAA1753] (STJA0020181)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$293.25 - $1,634.61

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Confocal imaging of NIH/3T3 cells using AP2A1 Rabbit monoclonal antibody (STJ11102071, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-AP2A1 antibody (1-100) [S1MR] (STJ11102071)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of extracts of various cell lines, using AP2A1 antibody (STJ28943) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AP2A1 antibody (1-260) (STJ28943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-AP2A1 antibody (STJA0008724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of HeLa cells using Alpha2-adaptin/AP2A2 Rabbit polyclonal antibody (STJ27344) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-AP2A2 antibody (590-939) (STJ27344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using Alpha2-adaptin/AP2A2 antibody (STJ11104629) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AP2A2 antibody (590-939) [S4629RM] (STJ11104629)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Anti-AP2A2 antibody (STJA0008820)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AP2B1 antibody (70-120 aa) (STJ195077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates, using AP2B1 Rabbit monoclonal antibody (STJ11105219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AP2B1 antibody (752-951 aa) [S5219RM] (STJ11105219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunohistochemistry analysis of paraffin-embedded rat brain using AP2B1 Rabbit polyclonal antibody (STJ22627) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-AP2B1 antibody (752-951) (STJ22627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AP2M1 antibody (100-180 aa) (STJ190156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of extracts of HeLa cells, using AP2M1 antibody (STJ115897) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AP2M1 antibody (134-433) (STJ115897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using AP2M1 Rabbit polyclonal antibody (STJ22628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-AP2M1 antibody (134-433) (STJ22628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunofluorescence analysis of U-251MG cells using AP2M1 Rabbit monoclonal antibody (STJ11101275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AP2M1 antibody (300-400) [S5MR] (STJ11101275)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-AP2M1 antibody [R05-1C5] (STJA0036095)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of lysates from Mouse brain, using AP2S1 Rabbit mAb (STJ11103654) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-AP2S1 antibody (1-100) [S4MR] (STJ11103654)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$163.53 - $719.67

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Western blot analysis of various lysates using AP2S1 Rabbit polyclonal antibody (STJ114160) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-AP2S1 antibody (1-142) (STJ114160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-AP2S1 antibody (26-76 aa) (STJ194906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-AP2S1 antibody (STJA0037487)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-AP2S1 antibody [R06-5T5] (STJA0037050)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/FC

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Western blot analysis of A549 cells using RIAM Polyclonal Antibody

Anti-APBB1IP antibody (430-510 aa) (STJ95456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse lung using 3 Mu g APC antibody (STJ11101456). Western blot was performed from the immunoprecipitate using APC antibody (STJ11101456) at a dilution of 1:500.

Anti-APC antibody (1-100) [S6MR] (STJ11101456)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunofluorescence analysis of PC-12 cells using APC antibody (STJ22635) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-APC antibody (2600-2760) (STJ22635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of Hela cell. 1, APC Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Galectin-3 monoclonal antibody (6G2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-APC antibody (2794-2843 aa) (STJ91623)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-APC antibody [PT1154R] (STJA0040000)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-APC antibody [R06-2E4] (STJA0035119)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

$184.23 - $388.47

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Anti-APC antibody [R08-6J7] (STJA0036008)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-APC antibody [SRM14480] (STJ11109210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Anti-APH1a antibody (90-140 aa) (STJ13100689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$445.05

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Immunofluorescence analysis of L929 cells using APH1A Rabbit polyclonal antibody (STJ116870) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-APH1A antibody (C-Term) (STJ116870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Anti-APH1A antibody (STJA0007460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot with Anti-APH1B Antibody (STJ500105) with peptide on at 1:500 dilution in buffer.

Anti-APH1B antibody (235-257) (STJ500105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

C.elegans/Human/Mouse/Rat

$552.69

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Anti-APH1B antibody (235-257) {Biotin} (STJ500106)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

C.elegans/Human/Mouse/Rat

$686.55

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Anti-APH1B antibody (235-257) {FITC} (STJ500107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

C.elegans/Human/Mouse/Rat

$686.55

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Western blot analysis of various lysates using APITD1 Rabbit pAb (STJ110592) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-APITD1 antibody (1-138) (STJ110592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunohistochemistry of rat small intestine with Anti-APLN primary antibody (STJ500102) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-APLN antibody (25-77) (STJ500102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$552.69

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Anti-APLN antibody (25-77) {Biotin} (STJ500103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-APLN antibody (25-77) {FITC} (STJ500104)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Western blot analysis of 22RV-1, H460, U87, mouse brain cells using Apelin Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-APLN antibody (28-77 aa) (STJ96528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-APLN/Apelin antibody [SAA1754] (STJA0020182)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$293.25 - $1,634.61

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Western blot analysis of 293 cells using APLNR Polyclonal Antibody

Anti-APLNR antibody (141-190 aa) (STJ91630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunohistochemistry of rat liver with Anti-APLNR primary antibody (STJ500111) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-APLNR antibody (150-200) (STJ500111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

$552.69

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Anti-APLNR antibody (150-200) {Biotin} (STJ500109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

$686.55

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Anti-APLNR antibody (150-200) {FITC} (STJ500110)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human

$686.55

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