Signal Transduction | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-MAP2K5 antibody (STJ196155)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, 3) PC12 using MEK-5 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MAP2K5 antibody (STJ197347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Mouse thymus, using MAP3K11 Rabbit polyclonal antibody (STJ118681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAP3K11 antibody (140-260) (STJ118681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using MLK3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MAP3K11 antibody (640-689) (STJ94151)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using TAK1 Rabbit polyclonal antibody (STJ113923) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAP3K7 antibody (110-325) (STJ113923)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, Tak1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CK7 monoclonal antibody (12D7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MAP3K7 antibody (161-210 aa) (STJ95901)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using TAK1 antibody (STJ117999). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-MAP3K7 antibody (510-606) (STJ117999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of TAK1 in A431 using MAP3K7 antibody.

Anti-MAP3K7 antibody (550-606 aa) [R09-4G4] (STJA0014935)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis of TAK1 in Hela, A431 lysates using TAK1 antibody.

Anti-MAP3K7 antibody (593-606 aa) [R09-4B6] (STJA0012028)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£264.50

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Western blot analysis of 3T3 cells using ERK 2 Polyclonal Antibody diluted at 1：2000

Anti-MAPK1 antibody (136-185 aa) (STJ92992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using [KO Validated] ERK2 Rabbit polyclonal antibody (STJ113009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAPK1 antibody (200-300) (STJ113009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (STJ24482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAPK1 antibody (200-300) (STJ24482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using ERK2 antibody (STJ11101738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK1 antibody (200-300) [S8MR] (STJ11101738)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot with Anti-MAPK1 Antibody (STJ501678) of mouse brain at 1:500 dilution in buffer. Second band may represent phosphorylated MAPK1.

Anti-MAPK1 antibody (300-350) (STJ501678)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK1 antibody (300-350) {Biotin} (STJ501679)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK1 antibody (300-350) {FITC} (STJ501680)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK1 antibody (C-Term) [M233] (STJA0005410)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot analysis using ERK 2 monoclonal antibody against HeLa (1) , NIH/3T3 (2) , MCF-7 (3) , HEK293 (4) , Jurkat (5) , A549 (6) , NTERA-2 (7) and SMMC-7721 (8) cell lysate.

Anti-MAPK1 antibody [4C11] (STJ98047)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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Western blot analysis using p38 Beta monoclonal antibody against truncated p38 Beta recombinant protein (1) and full-length p38 Beta (aa1-363) -pcDNA3.1 transfected CHO-K1 cell lysate (2).

Anti-MAPK11 antibody (251-363 aa) [4H6H6] (STJ98300)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of PC-12 cells using MAPK11 Rabbit polyclonal antibody (STJ110028) at a dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-MAPK11 antibody (264-364) (STJ110028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot with Anti-MAPK11 Antibody (STJ501684) of 1) Rat cortex, 2) Mouse cerebellum, 3) Mouse lung, 4) HEK cells. Antibody is at 1:500 dilution in buffer.

Anti-MAPK11 antibody (300-350) (STJ501684)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK11 antibody (300-350) {Biotin} (STJ501685)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK11 antibody (300-350) {FITC} (STJ501686)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various lysates using MAPK12 Rabbit polyclonal antibody (STJ115013) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAPK12 antibody (248-367) (STJ115013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry of mouse muscle with Anti-MAPK12 primary antibody (STJ501687) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-MAPK12 antibody (310-360) (STJ501687)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK12 antibody (310-360) {Biotin} (STJ501688)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK12 antibody (310-360) {FITC} (STJ501689)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MAPK12 antibody (70-150 aa) (STJ191718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-MAPK12 antibody (STJA0010266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using MAPK13 antibody (STJ29632) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-MAPK13 antibody (1-365) (STJ29632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-MAPK13 antibody (200-280 aa) (STJ191719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using MAPK13 Rabbit polyclonal antibody (STJ11102816) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MAPK13 antibody (300-400) (STJ11102816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry with Anti-MAPK13 Antibody (STJ501690) at 1:100 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-MAPK13 antibody (310-360) (STJ501690)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK13 antibody (310-360) {Biotin} (STJ501691)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK13 antibody (310-360) {FITC} (STJ501692)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK13 nanobody [SAA1368] (STJN000042)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF/IP

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of extracts of various cell lines, using p38 MAPK rabbit monoclonal antibody (STJ11102152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-MAPK14 antibody (100-200) [S2MR] (STJ11102152)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, p38 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-MAPK14 antibody (147-196 aa) (STJ94878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Fish/Pig

£48.00 - £252.00

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Immunohistochemistry with Anti-MAPK14 Antibody (STJ501693) at 1:100 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000. FFPE section.

Anti-MAPK14 antibody (150-200) (STJ501693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK14 antibody (150-200) {Biotin} (STJ501694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK14 antibody (150-200) {FITC} (STJ501695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of extracts of various cell lines, using p38 MAPK antibody (STJ112728) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-MAPK14 antibody (261-360) (STJ112728)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £642.50

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Western blot analysis of extracts of various cell lines, using p38 MAPK antibody (STJ113086) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK14 antibody (261-360) (STJ113086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of U-87MG cells, using 3 Mu g p38 MAPK antibody (STJ116613). Western blot was performed from the immunoprecipitate using p38 MAPK antibody (STJ116613) at a dilution of 1:1000.

Anti-MAPK14 antibody (261-360) (STJ116613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using p38 MAPK Rabbit polyclonal antibody (STJ27472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAPK14 antibody (261-360) (STJ27472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates of HepG2 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-p38 (PTR2306) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2Predicted band size: 41kDaObserved band size: 38kDa

Anti-MAPK14 antibody (300-360 aa) [4G2] (STJ196672)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of HELA KB PC-3 using p38 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-MAPK14 antibody (C-Term) (STJ97615)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£48.00 - £252.00

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Anti-Mapk14 antibody (C-Term) [M548] (STJA0005504)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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