• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g HDAC6 antibody (STJ11103524). Western blot was performed from the immunoprecipitate using HDAC6 antibody (STJ11103524) at a dilution of 1:1000.
  • Immunohistochemistry analysis of HDAC6 in paraffin-embedded human breast cancer using HDAC6 Rabbit monoclonal antibody (STJ11103524) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HDAC6 Rabbit monoclonal antibody (STJ11103524) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HDAC6 antibody (1-100) [S4MR] (STJ11103524)

SKU:
STJ11103524

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HDAC6 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC6
Gene ID: 10013
Uniprot ID: HDAC6_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC6 (Q9UBN7).
Immunogen Sequence: MTSTGQDSTTTRQRRSRQNP QSPPQDSSVTSKRNIKKGAV PRSIPNLAEVKKKGKMKKLG QAMEEDLIVGLQGMDLNLEA EALAGTGLVLDEQLNEFHCL
Post Translational Modifications Phosphorylated by AURKA.phosphorylation increases HDAC6-mediated deacetylation of alpha-tubulin and subsequent disassembly of cilia. Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro.
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. In addition to histones, deacetylates other proteins: plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin. Required for cilia disassembly.via deacetylation of alpha-tubulin. Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Promotes odontoblast differentiation following IPO7-mediated nuclear import and subsequent repression of RUNX2 expression. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy.
Protein Name Histone Deacetylase 6
Hd6
Tubulin-Lysine Deacetylase Hdac6
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-3371511
Reactome: R-HSA-350054
Reactome: R-HSA-5617833
Reactome: R-HSA-8878166
Reactome: R-HSA-8940973
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9646399
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Perikaryon
Cell Projection
Dendrite
Axon
Cilium
Microtubule Organizing Center
Centrosome
Cilium Basal Body
It Is Mainly Cytoplasmic
Where It Is Associated With Microtubules
Alternative Antibody Names Anti-Histone Deacetylase 6 antibody
Anti-Hd6 antibody
Anti-Tubulin-Lysine Deacetylase Hdac6 antibody
Anti-HDAC6 antibody
Anti-KIAA0901 antibody
Anti-JM21 antibody

Information sourced from Uniprot.org

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