• Western blot analysis of extracts of various cell lines, using HDAC8 rabbit monoclonal antibody (STJ11102387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g HDAC8 antibody (STJ11102387). Western blot was performed from the immunoprecipitate using HDAC8 antibody (STJ11102387) at a dilution of 1:1000.
  • Western blot analysis of extracts of various cell lines, using HDAC8 rabbit monoclonal antibody (STJ11102387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Confocal imaging of HeLa cells using [KO Validated] HDAC8 Rabbit monoclonal antibody (STJ11102387, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Western blot analysis of extracts from normal (control) and HDAC8 knockout (KO) 293T cells, using HDAC8 antibody (STJ11102387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of HDAC8 in paraffin-embedded rat brain using HDAC8 Rabbit monoclonal antibody (STJ11102387) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using HDAC8 rabbit monoclonal antibody (STJ11102387) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and HDAC8 knockout (KO) 293T cells, using [KO Validated] HDAC8 Rabbit monoclonal antibody (STJ11102387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded human liver cancer using HDAC8 rabbit monoclonal antibody (STJ11102387) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HDAC8 Rabbit monoclonal antibody (STJ11102387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunohistochemistry of paraffin-embedded mouse testis using HDAC8 rabbit monoclonal antibody (STJ11102387) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HDAC8 Rabbit monoclonal antibody (STJ11102387) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of NIH-3T3 cells using HDAC8 rabbit monoclonal antibody (STJ11102387) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HDAC8 antibody (STJ11102387). Western blot was performed from the immunoprecipitate using HDAC8 antibody (STJ11102387) at a dilition of 1:1000.

Anti-HDAC8 antibody (1-100) [S7MR] (STJ11102387)

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STJ11102387

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-HDAC8 (1-100) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HDAC8
Gene ID: 55869
Uniprot ID: HDAC8_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC8 (Q9BY41).
Immunogen Sequence: MEEPEEPADSGQSLVPVYIY SPEYVSMCDSLAKIPKRASM VHSLIEAYALHKQMRIVKPK VASMEEMATFHTDAYLQHLQ KVSQEGDDDHPDSIEYGLGY
Tissue Specificity Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney.
Post Translational Modifications Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4.
Function Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility. In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones.
Protein Name Histone Deacetylase 8
Hd8
Protein Deacetylase Hdac8
Protein Decrotonylase Hdac8
Database Links Reactome: R-HSA-2122947
Reactome: R-HSA-2467813 Q9BY41-1
Reactome: R-HSA-2500257 Q9BY41-1
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-3214815
Reactome: R-HSA-350054
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Excluded From The Nucleoli
Found In The Cytoplasm Of Cells Showing Smooth Muscle Differentiation
Alternative Antibody Names Anti-Histone Deacetylase 8 antibody
Anti-Hd8 antibody
Anti-Protein Deacetylase Hdac8 antibody
Anti-Protein Decrotonylase Hdac8 antibody
Anti-HDAC8 antibody
Anti-HDACL1 antibody
Anti-CDA07 antibody

Information sourced from Uniprot.org

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