Signal Transduction | Research Area | St John's Laboratory

Immunofluorescence analysis of PC-12 cells using IRS1 Rabbit polyclonal antibody (STJ24233) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-IRS1 antibody (1043-1242) (STJ24233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of extracts of HepG2 cells, using IRS1 antibody (STJ11101611) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-IRS1 antibody (1100-1200) [S1MR] (STJ11101611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

Choose Options

Immunofluorescence analysis of human-lung tissue. 1, IRS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-IRS1 antibody (603-652 aa) (STJ93769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from HepG2 cells, using IRS1 Rabbit pAb (STJ112968) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-IRS1 antibody (735-855) (STJ112968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates, using IRS2 Rabbit pAb (STJ110254) at 1:900 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-IRS2 antibody (1238-1338) (STJ110254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of HEPG2, HELA cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000

Anti-IRS2 antibody (660-700 aa) (STJ98667)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Anti-IRS4 antibody (STJA0004313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human

£140.50 - £415.50

Choose Options

Western blot analysis of various lysates using ITCH Rabbit mAb (STJ11102343) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ITCH antibody (1-100) [S3MR] (STJ11102343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunohistochemistry analysis of ITCH in paraffin-embedded mouse brain using ITCH Rabbit polyclonal antibody (STJ11103875) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ITCH antibody (1-155) (STJ11103875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from wild type (WT) and ITCH knockout (KO) 293T cells, using [KO Validated] ITCH Rabbit polyclonal antibody (STJ118061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ITCH antibody (1-155) (STJ118061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Western blot with Anti-ITCH Antibody (STJ501506) of THP cell at 1:500 dilution in buffer.

Anti-ITCH antibody (250-300) (STJ501506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

Choose Options

Anti-ITCH antibody (250-300) {Biotin} (STJ501507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-ITCH antibody (250-300) {FITC} (STJ501508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Western blot analysis of lysates from mouse brain, using ITCH Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ITCH antibody (386-435 aa) (STJ91517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of NIH-3T3 cells using Integrin Alpha 2 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ITGA2 antibody (1081-1130 aa) (STJ97283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunohistochemistry analysis of Integrin alpha 2 (ITGA2/CD49b) in paraffin-embedded human colon carcinoma using Integrin alpha 2 (ITGA2/CD49b) (ITGA2/CD49b) Rabbit monoclonal antibody (STJ11101605) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ITGA2 antibody (1082-1181) [S5MR] (STJ11101605)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Integrin alpha 2 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-ITGA2 antibody (1100-1181 aa) [R09-7C3] (STJA0014704)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£264.50

Choose Options

Immunofluorescence analysis of U2OS cells using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ITGA2 antibody (30-162) (STJ29766)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Immunohistochemistry analysis of CD41/ITGA2B in paraffin-embedded human appendix using CD41/ITGA2B Rabbit monoclonal antibody (STJ11101345) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ITGA2B antibody (1-100) [S5MR] (STJ11101345)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunofluorescence analysis of HEL cells using CD41/ITGA2B Rabbit polyclonal antibody (STJ11103826) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ITGA2B antibody (160-260) (STJ11103826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from mouse liver, using CD41/ITGA2B Rabbit pAb (STJ27647) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ITGA2B antibody (693-993) (STJ27647)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Anti-ITGA2B antibody (STJA0010141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, CD41 monoclonal antibody (Q90) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-ITGA2B antibody [Q90] (STJ97008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£48.00 - £252.00

Choose Options

Western blot analysis of various cells using Integrin Alpha 3 Polyclonal Antibody diluted at 1：2000

Anti-ITGA3 antibody (482-531 aa) (STJ93727)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates using ITGA3 Rabbit polyclonal antibody (STJ119594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ITGA3 antibody (85-260) (STJ119594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of extracts from wild type (WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ11104927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ITGA5 antibody (42-995 aa) [S4927RM] (STJ11104927)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

Choose Options

Western blot analysis of extracts of various cell lines, using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ11104662) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ITGA5 antibody (42-995) (STJ11104662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Western blot analysis of Hela cells using Integrin Alpha 5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ITGA5 antibody (561-610 aa) (STJ97284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of Human placenta using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ27965) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ITGA5 antibody (750-1000) (STJ27965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis using Integrin Alpha 5 monoclonal antibody against HeLa, HUVEC cell lysate.

Anti-ITGA5 antibody (N-Term) (STJ98510)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC

Reactivity :

Human/Mouse/Rat/Cow

£144.00 - £348.00

Choose Options

Western blot analysis using Integrin Alpha 5 monoclonal antibody against ITGA5-hIgGFc transfected HEK293 cell lysate.

Anti-ITGA5 antibody [10F6] (STJ98181)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Western blot analysis of various lysates using ITGA8 Rabbit polyclonal antibody (STJ115023) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ITGA8 antibody (907-1000) (STJ115023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-ITGA8 antibody [SAA1983] (STJA0020411)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of C6 cells using 3 Mu g Integrin alpha V (ITGAV/CD51) antibody (STJ24252). Western blot was performed from the immunoprecipitate using Integrin alpha V (ITGAV/CD51) antibody (STJ24252) at a dilution of 1:2000.

Anti-ITGAV antibody (1021-1034) (STJ24252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunocytochemistry analysis of Integrin alpha V (green) in A549 using Integrin alpha V antibody, and DAPI (blue).

Anti-ITGAV antibody (32-236 aa) [R02-6G3] (STJA0010733)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£264.50

Choose Options

Western blot analysis of various cells using Integrin Alpha V Polyclonal Antibody diluted at 1：1000

Anti-ITGAV antibody (755-804 aa) (STJ93730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using Integrin alpha V (ITGAV/CD51) (ITGAV/CD51) antibody (STJ11101608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ITGAV antibody (949-1048) [S8MR] (STJ11101608)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Immunofluorescence analysis of NIH-3T3 cells using Integrin-Beta 1/CD29 Rabbit polyclonal antibody (STJ111129) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ITGB1 antibody (1-728) (STJ111129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from Jurkat cells, using Integrin beta1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ITGB1 antibody (746-795 aa) (STJ93732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-ITGB1 antibody (Extracellular region) [M004] (STJA0005446)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£390.50

Choose Options

Anti-ITGB1 antibody (Extracellular region) [M006] (STJA0005447)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£390.50

Choose Options

Anti-ITGB1 antibody (Extracellular region) [M032] (STJA0005448)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£365.50

Choose Options

Anti-ITGB1 antibody (Extracellular region) [M041] (STJA0005449)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£390.50

Choose Options

Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD61 (ABT032) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HUVECPredicted band size: 70kDaObserved band size: 70kDa

Anti-ITGB3 antibody (1-100 aa) [PT0052] (STJ196789)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

Choose Options

Immunohistochemistry of baboon kidney with Anti-ITGB3 primary antibody (STJ501482) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-ITGB3 antibody (1-50) (STJ501482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£427.50

Choose Options

Anti-ITGB3 antibody (1-50) {Biotin} (STJ501483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

Choose Options

Anti-ITGB3 antibody (1-50) {FITC} (STJ501484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

Choose Options

Western blot analysis of various lysates using ITGB3 Rabbit polyclonal antibody (STJ11105487) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB3 antibody (21-718) (STJ11105487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options