Neuronal System | Research Area | St John's Laboratory

Anti-Phospho-KCNC4-Ser15 antibody (STJ91317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-KCND2-Ser616 antibody (STJ91165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using ROMK/Kir1.1 (Phospho-Ser44/25) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-KCNJ1-Ser44 antibody (11-60 aa) (STJ91320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-KCNJ11-Thr224 antibody (190-239 aa) (STJ90722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using GIRK1/KIR3.1/KCNJ3 (Phospho-Ser185) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-KCNJ3-Ser185 antibody (151-200 aa) (STJ91092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-Phospho-MAPK1 antibody [M206] (STJA0005411)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ERK1-Y204 + ERK2-Y187 Rabbit pAb (STJ22262) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAPK3-Y204 antibody (STJ22262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from 293 cells, using Phospho-MDM2-S166 Rabbit pAb (STJ22283). 293 cells were treated by Hydroxyurea (4mM) for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MDM2-S166 antibody (STJ22283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded rat ovary using Phospho-MDM2-S166 Rabbit monoclonal antibody (STJ11105811) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MDM2-S166 antibody [S5811RM] (STJ11105811)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from 293 cells, using Phospho-MDM2-S260 Rabbit pAb (STJ22284). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-MDM2-S260 antibody (STJ22284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, MDM2 (phospho Ser166) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HAO1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-MDM2-Ser166 antibody (132-181 aa) (STJ91043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using MDM2 (Phospho-Ser186+Ser188) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MDM2-Ser186/S188 antibody (151-200 aa) (STJ91352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of HELA-UV Cell Lysate using antibody

Anti-Phospho-MDM2-Tyr394 antibody (STJ98946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-NMDAR1-S896/S897 Rabbit pAb (STJ11103722) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 30 minutes and PMA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-NMDAR1-S896/S897 antibody (STJ11103722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-NMDAR1-S897 antibody (STJ11107680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of HELA cells using Phospho-Trk C (Y516) Polyclonal Antibody

Anti-Phospho-NTRK3-Tyr516 antibody (482-531 aa) (STJ90960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HUVEC cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S352 Rabbit pAb (STJ11107616). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-p90Rsk/RSK1/RPS6KA1-S352 antibody (STJ11107616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-p90Rsk/RSK1/RPS6KA1-T573 antibody (STJ11107649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-PDK1-Ser-241 antibody (STJA0005522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-PDK1-Tyr-9 antibody (STJA0005521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of K562 cells using Phospho-PDLIM5 (Y251) Polyclonal Antibody

Anti-Phospho-PDLIM5-Tyr251 antibody (STJ90383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 Rabbit pAb (STJ22329) at 1:1000 dilution. NIH/3T3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-PDPK1-S241 antibody (STJ22329)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDK1/PDPK1-S241 pAb (STJ29323) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PDPK1-S241 antibody (STJ29323)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates, using Phospho-PDK1/PDPK1-S241 antibody (STJ11105841) at1:2000 dilution. PC-3 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Anti-Phospho-PDPK1-S241 antibody [S5841RM] (STJ11105841)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PDK1 (Phospho-Ser241) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PDPK1-Ser241 antibody (210-259 aa) (STJ90382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-PDPK1-Ser241 antibody [PT0869R] (STJA0039151)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemistry analysis of paraffin-embedded human brain, using PDK1 (Phospho-Tyr9) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PDPK1-Tyr9 antibody (1-50 aa) (STJ91192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-PLC beta 3 (PLCB3) -S1105 Rabbit pAb (STJ11100978) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PLCB3-S1105 antibody (STJ11100978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-PLC Beta 3 (S1105) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PLCB3-Ser1105 antibody (1071-1120 aa) (STJ90766)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-PLC Beta 3 (S537) Polyclonal Antibody diluted at 1：500

Anti-Phospho-PLCB3-Ser537 antibody (503-552 aa) (STJ90867)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from C6 cells using Phospho-AMPK Alpha 1-S485 Rabbit pAb (STJ11101009) at 1:2000 dilution. C6 cells were treated by Lambda pp (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-PRKAA1-S485 antibody (STJ11101009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from C2C12 cells, using Phospho-AMPK Alpha 1-S496 Rabbit mAb (STJ11102591) at 1:1000 dilution. C2C12 cells were treated by oligomycin (0. 5 Mu M) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-PRKAA1-S496 antibody [S1MR] (STJ11102591)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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Western blot analysis of lysates from HeLa cells treated with heat shock, using AMPK1 (Phospho-Ser485) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKAA1-Ser485 antibody (STJ196239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HELA cells using Phospho-AMPK Alpha 1 (S496) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-PRKAA1-Ser496 antibody (451-500 aa) (STJ90170)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Dog/Fish

£48.00 - £252.00

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Western blot analysis of lysates from 293 cells, using Phospho-AMPK Beta 1-S108 Rabbit pAb (STJ29363) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKAB1-S108 antibody (STJ29363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-AMPK Beta 1 (S182) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-PRKAB1-Ser182 antibody (147-196 aa) (STJ90171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-PRKAB1-Ser182 antibody (147-196 aa) (STJ90874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from HeLa and 293 cells, using Phospho-PKA C-alpha (PRKACA) -S339 Rabbit pAb (STJ113496) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKACA-S339 antibody (STJ113496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-PRKACA-Ser338 antibody (STJ91123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 293T cells, using Phospho-PKA C-alpha (PRKACA) -T197 Rabbit pAb (STJ113495) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKACA-T197 antibody (STJ113495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-PKA RII Alpha (PRKAR2A) -S99 Rabbit mAb (STJ11102613) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PRKAR2A-S99 antibody [S3MR] (STJ11102613)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-PRKAR2B-Ser113 antibody (79-128 aa) (STJ90520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from 293 cells, using Phospho-PKC alpha-S226 Rabbit pAb (STJ113497) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKCA-S226 antibody (STJ113497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-PKC alpha-S319 Rabbit pAb (STJ117877) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight or treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PRKCA-S319 antibody (STJ117877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-PKC alpha-S657/Y658 Rabbit pAb (STJ11101090) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PRKCA-S657/Y658 antibody (STJ11101090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-PRKCA-S657/Y658 antibody (STJA0005533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Rabbit/Rat

£367.50

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Western blot analysis of 293T cells, using Phospho-PKC alpha-T638 antibody (STJ11105853) at 1:7000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-PRKCA-T638 antibody [S5853RM] (STJ11105853)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from C2C12 cells, using Phospho-PKC alpha-T638 Rabbit mAb (STJ11102617) at 1:1000 dilution. C2C12 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PRKCA-T638 antibody [S7MR] (STJ11102617)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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