Anti-Nicotinic Acetylcholine Receptor alpha 4 antibody [R03-1A5] (STJA0035289)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human Nicotinic Acetylcholine Receptor alpha 4/CHRNA4
General Information
| Short Description | Rabbit monoclonal anti-Nicotinic Acetylcholine Receptor alpha 4 for use in WB and IP in Human, Mouse, Rat and Hamster samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Hamster |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R03-1A5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CHRNA4 |
| Gene ID | 1137 |
| Uniprot ID | ACHA4_HUMAN |
| Immunogen | A synthetic peptide of human Nicotinic Acetylcholine Receptor alpha 4/CHRNA4 |
Additional Info
| Function | Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators. CHRNA4 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, as well as CHRNA5 and CHRNB3 as accesory subunits. Is the most abundant nAChR subtype expressed in the central nervous system. Found in two major stoichiometric forms,(CHRNA4)3:(CHRNB2)2 and (CHRNA4)2:(CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation. Involved in the modulation of calcium-dependent signaling pathways, influences the release of neurotransmitters, including dopamine, glutamate and GABA. |
| Protein Name | Neuronal Acetylcholine Receptor Subunit Alpha-4 |
| Database Links | Reactome: R-HSA-629587Reactome: R-HSA-629594Reactome: R-HSA-629597 |
| Cellular Localisation | Synaptic Cell MembraneMulti-Pass Membrane ProteinCell Membrane |
| Alternative Antibody Names | Anti-Neuronal Acetylcholine Receptor Subunit Alpha-4 antibodyAnti-CHRNA4 antibodyAnti-NACRA4 antibody |
Information sourced from Uniprot.org