• Western blot analysis of extracts of various cell lines, using Human Neurogranin antibody (STJ11104673) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NRGN antibody (2-78) (STJ11104673)

SKU:
STJ11104673

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Neurogranin (2-78) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NRGN
Gene ID: 4900
Uniprot ID: NEUG_HUMAN
Immunogen Region: 2-78
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-78 of human Human Neurogranin (NP_001119653.1).
Immunogen Sequence: DCCTENACSKPDDDILDIPL DDPGANAAAAKIQASFRGHM ARKKIKSGERGRKGPGPGGP GGAGVARGGAGGGPSGD
Tissue Specificity In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease.
Post Translational Modifications Phosphorylated at Ser-36 by PHK and PKC, phosphorylation prevents interaction with Calmodulin and interrupts several learning- and memory-associated functions.
Function Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.
Protein Name Neurogranin
Ng
Rc3 Cleaved Into - Neug(55-78
Database Links Reactome: R-HSA-9620244
Alternative Antibody Names Anti-Neurogranin antibody
Anti-Ng antibody
Anti-Rc3 Cleaved Into - Neug(55-78 antibody
Anti-NRGN antibody

Information sourced from Uniprot.org

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