Neuronal System | Research Area | St John's Laboratory

Western blot analysis of extracts from normal (control) and ERK2 knockout (KO) HeLa cells, using ERK2 antibody (STJ24482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAPK1 antibody (200-300) (STJ24482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using ERK2 antibody (STJ11101738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK1 antibody (200-300) [S8MR] (STJ11101738)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot with Anti-MAPK1 Antibody (STJ501678) of mouse brain at 1:500 dilution in buffer. Second band may represent phosphorylated MAPK1.

Anti-MAPK1 antibody (300-350) (STJ501678)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK1 antibody (300-350) {Biotin} (STJ501679)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK1 antibody (300-350) {FITC} (STJ501680)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK1 antibody (C-Term) [M233] (STJA0005410)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot analysis using ERK 2 monoclonal antibody against HeLa (1) , NIH/3T3 (2) , MCF-7 (3) , HEK293 (4) , Jurkat (5) , A549 (6) , NTERA-2 (7) and SMMC-7721 (8) cell lysate.

Anti-MAPK1 antibody [4C11] (STJ98047)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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Western blot analysis of various lysates using ERK1 Rabbit monoclonal antibody (STJ11104593) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-MAPK3 antibody (1-100) [S4593RM] (STJ11104593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various lysates, using ERK1 antibody (STJ11101698) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MAPK3 antibody (1-100) [S8MR] (STJ11101698)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of ERK1 in paraffin-embedded mouse heart using [KD Validated] ERK1 Rabbit polyclonal antibody (STJ24487) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-MAPK3 antibody (1-378) (STJ24487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using ERK 1 Polyclonal Antibody diluted at 1：500

Anti-MAPK3 antibody (161-210 aa) (STJ92987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 using ERK1 Rabbit monoclonal antibody (STJ11104626) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK3 antibody (200-300) [S4626RM] (STJ11104626)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Rat

£129.50 - £574.50

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Western blot with Anti-MAPK3 Antibody (STJ501702) of rat testis at 1:250 dilution in 1% milk solution.

Anti-MAPK3 antibody (300-350) (STJ501702)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-MAPK3 antibody (300-350) {Biotin} (STJ501703)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-MAPK3 antibody (300-350) {FITC} (STJ501704)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-MAPK3 antibody (STJ197394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using ERK 1 monoclonal antibody against HeLa (1) , Jurkat (2) , RAW264.7 (3) , HEK293 (4) , K562 (5) , NIH/3T3 (6) , Cos7 (7) and PC-12 (8) cell lysate.

Anti-MAPK3 antibody [1E5] (STJ98045)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of rat-spleen tissue. 1, ERK1 Mouse monoclonal antibody (5E9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-MAPK3 antibody [5E9] (STJ97780)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of MDM2 in paraffin-embedded human lung squamous carcinoma tissue using MDM2 Rabbit monoclonal antibody (STJ11105095) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MDM2 antibody (101-200) [S5095RM] (STJ11105095)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of lysates from U2OS cells, using MDM2 Rabbit monoclonal antibody (STJ11105096) at 1:2000 dilution. U2OS cells were treated by Nutlin 3a (10 Mu M) at 37 °C for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MDM2 antibody (101-200) [S5096RM] (STJ11105096)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunofluorescence analysis of Hela cell. 1, MDM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3B Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-MDM2 antibody (132-181 aa) (STJ94057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells, using MDM2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MDM2 antibody (151-200 aa) (STJ94056)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Human atypical lipomatous tumor stained with anti-MDM2 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells.

Anti-MDM2 antibody (154-167aa) [SMP14] (STJ180166)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£163.50 - £592.50

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Western blot analysis of C2C12, using MDM2 antibody (STJ115290) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MDM2 antibody (200-430) (STJ115290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human urothelial carcinoma using MDM2 Rabbit polyclonal antibody (STJ24533) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-MDM2 antibody (231-330) (STJ24533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of human-liver-cancer tissue. 1, MDM2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-MDM2 antibody (381-430 aa) (STJ96551)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of A549 cells, using MDM2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MDM2 antibody (391-440 aa) (STJ94055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-MDM2 antibody [PT0681R] (STJA0039292)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-MDM2 antibody [SMP14] (STJ16101854)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/WB

Reactivity :

Human/Mouse/Rat

£869.50

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Western blot analysis of extracts from Jurkat cells, using Myosin VI Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-MYO6 antibody (40-120 aa) (STJ96437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NAAA antibody (230-310 aa) (STJ192408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-NBEA antibody (STJA0004614)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Western blot analysis of various lysates using NCALD Rabbit pAb (STJ110307) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-NCALD antibody (1-193) (STJ110307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-NCALD antibody (370-450) (STJ94428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Human appendix tissue was stained with Anti-Neurofilament (ABT208) Antibody

Anti-NEFL antibody (400-543 aa) [ABT208] (STJ197175)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Human appendix tissue was stained with Anti-Neurofilament (ABT356) Antibody

Anti-NEFL antibody (400-543 aa) [ABT356] (STJ197176)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded mouse brain using Neurofilament L Rabbit polyclonal antibody (STJ24733) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NEFL antibody (60-250) (STJ24733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts from Jurkat cells, using NF-L Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-NEFL antibody (C-Term) (STJ96461)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Formalin-fixed, paraffin-embedded human cerebellum stained with anti-neurofilament antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of neurons and neuronal processes

Anti-Neurofilament antibody [ZR216] (STJ180529)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£137.50 - £398.50

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Confocal imaging of paraffin-embedded Mouse brain tissue using Neurofilament L Rabbit monoclonal antibody (STJ11103250, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Neurofilament L antibody (60-250) [S0MR] (STJ11103250)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using Neurogranin (NRGN) Rabbit mAb (STJ11107284) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Enhanced Kit <br>Negative control (NC) : Mouse lung<br>Exposure time: 45s.

Anti-Neurogranin antibody (2-78 aa) [SRM] (STJ11107284)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Anti-NLGN1 antibody (410-490 aa) (STJ191069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-NLGN2 antibody (15-180 aa) (STJ11106122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-NLGN2 antibody (310-390 aa) (STJ191070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-NLGN3 antibody (610-690 aa) (STJ191071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NLGN4X antibody (601-651 aa) (STJ193961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human prostate using NLGN4Y antibody (STJ26706) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-NLGN4Y antibody (122-256) (STJ26706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-NLGN4Y antibody (STJA0009352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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