Anti-KCNK3 antibody (250-300 aa) (STJ13101279)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 250-300 of human KCNK3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
General Information
| Short Description | Rabbit polyclonal anti-KCNK3 (250-300 aa) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | KCNK3 |
| Gene ID | 3777 |
| Uniprot ID | KCNK3_HUMAN |
| Immunogen | A synthetic peptide from aa region 250-300 of human KCNK3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Immunogen Region | 250-300 aa |
| Specificity | Specific for KCNK3. |
Additional Info
| Function | K(+) channel that conducts voltage-dependent outward rectifying currents upon membrane depolarization. Voltage sensing is coupled to K(+) electrochemical gradient in an 'ion flux gating' mode where outward but not inward ion flow opens the gate. Changes ion selectivity and becomes permeable to Na(+) ions in response to extracellular acidification. Protonation of the pH sensor His-98 stabilizes C-type inactivation conformation likely converting the channel from outward K(+)-conducting, to inward Na(+)-conducting to nonconductive state. Homo- and heterodimerizes to form functional channels with distinct regulatory and gating properties. Allows K(+) currents with fast-gating kinetics important for the repolarization and hyperpolarization phases of action potentials. In cerebellar granule cells, heteromeric KCNK3:KCNK9 channel may hyperpolarize the resting membrane potential to limit intrinsic neuronal excitability, but once the action potential threshold is reached, it may support high-frequency action potential firing and increased neuronal excitability. Dispensable for central chemosensory respiration i.e. breathing controlled by brainstem CO2/pH, it rather conducts pH-sensitive currents and controls the firing rate of serotonergic raphe neurons involved in potentiation of the respiratory chemoreflex. Additionally, imparts chemosensitivity to type 1 cells in carotid bodies which respond to a decrease in arterial oxygen pressure or an increase in carbon dioxide pressure or pH to initiate adaptive changes in pulmonary ventilation. In adrenal gland, contributes to the maintenance of a hyperpolarized resting membrane potential of aldosterone-producing cells at zona glomerulosa and limits aldosterone release as part of a regulatory mechanism that controls arterial blood pressure and electrolyte homeostasis. In brown adipocytes, mediates K(+) efflux that counteracts norepinephrine-induced membrane depolarization, limits Ca(2+) efflux and downstream cAMP and PKA signaling, ultimately attenuating lipid oxidation and adaptive thermogenesis. |
| Protein Name | Potassium Channel Subfamily K Member 3Acid-Sensitive Potassium Channel Protein Task-1Twik-Related Acid-Sensitive K(+ Channel 1Two Pore Potassium Channel Kt3.1Two Pore K(+ Channel Kt3.1 |
| Database Links | Reactome: R-HSA-1299316Reactome: R-HSA-5576886 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Potassium Channel Subfamily K Member 3 antibodyAnti-Acid-Sensitive Potassium Channel Protein Task-1 antibodyAnti-Twik-Related Acid-Sensitive K(+ Channel 1 antibodyAnti-Two Pore Potassium Channel Kt3.1 antibodyAnti-Two Pore K(+ Channel Kt3.1 antibodyAnti-KCNK3 antibodyAnti-TASK antibodyAnti-TASK1 antibody |
Information sourced from Uniprot.org