Metabolism | Research Area | St John's Laboratory

Western blot analysis of lysates from HepG2 cells, using MAT2A Rabbit monoclonal antibody (STJ11103003) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-MAT2A antibody (1-100) [S3MR] (STJ11103003)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Western blot analysis of various lysates using MAT2A Rabbit pAb (STJ110734) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-MAT2A antibody (1-395) (STJ110734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MAT2A antibody (294-344 aa) (STJ195504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of MAT2A in (1) Ramos lysates; (2) mouse spleen lysates using MAT2A antibody.

Anti-MAT2A antibody (50-138 aa) [R07-5R5] (STJA0015646)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

€330.63

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Western blot analysis of various lysates using MAT2B Rabbit polyclonal antibody (STJ113213) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MAT2B antibody (1-334) (STJ113213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MAT2B antibody (23-73 aa) (STJ193913)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-MBOAT1 antibody (150-200) (STJ507771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

€500.63

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Anti-MBOAT1 antibody (150-200) {Biotin} (STJ507772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

€621.88

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Anti-MBOAT1 antibody (150-200) {FITC} (STJ507773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

€621.88

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MBOAT7 antibody (293-343 aa) (STJ194205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MBTPS1 antibody (180-260 aa) (STJ95674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various lysates using MBTPS1 Rabbit polyclonal antibody (STJ118682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MBTPS1 antibody (803-1052) (STJ118682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Immunofluorescence analysis of A549 cells, using MBTPS2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MBTPS2 antibody (301-350 aa) (STJ95570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from LO2 cells, using MCAT Rabbit polyclonal antibody (STJ118267) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MCAT antibody (191-390) (STJ118267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MCCC1 antibody (620-670 aa) (STJ195538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of T-47D, using MCCC1 rabbit polyclonal antibody (STJ112060) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MCCC1 antibody (640-725) (STJ112060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-MCCC1 antibody [SRM14795] (STJ11109525)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MCCC2 antibody (204-254 aa) (STJ193568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various lysates using MCCC2 Rabbit pAb (STJ110297) at 1:2000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Negative control (NC) : MOLT-4. <br/>Exposure time: 30s.

Anti-MCCC2 antibody (284-563) (STJ110297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunofluorescence analysis of U2OS cells using MCCC2 antibody (STJ117375) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-MCCC2 antibody (284-563) (STJ117375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-MCCC2 antibody [SRM14919] (STJ11109649)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of various lysates using MCEE Rabbit polyclonal antibody (STJ116640) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-MCEE antibody (1-176) (STJ116640)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Anti-MCEE antibody (STJA0008245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of extracts of various cell lines, using MDH1 antibody (STJ29701) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MDH1 antibody (1-334) (STJ29701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various lysates using MDH1 Rabbit mAb (STJ11102525) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-MDH1 antibody (235-334) [S5MR] (STJ11102525)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MDH1 antibody (STJ193110)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of extracts of various cell lines, using MDH2 antibody (STJ115477) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MDH2 antibody (59-338) (STJ115477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Anti-MDH2 antibody (STJA0006978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-MDH2 antibody [SRM13132] (STJ11107862)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Anti-MDR3 antibody [IHC621] (STJA0042048)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

€285.63 - €1,065.63

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Western blot analysis of extracts of various cell lines, using ME1 antibody (STJ24535) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ME1 antibody (1-300) (STJ24535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of lysates from HeLa cells, using ME1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ME1 antibody (468-517 aa) (STJ93994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of extracts from wild type (WT) and ME1 knockdown (KD) MCF7 (KD) cells, using ME1 antibody (STJ11105005) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ME1 antibody (472-572) (STJ11105005)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using ME2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ME2 antibody (201-250 aa) (STJ94059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of various lysates using ME2 Rabbit pAb (STJ111777) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ME2 antibody (220-479) (STJ111777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Immunofluorescence analysis of L929 cells using ME3 Rabbit polyclonal antibody (STJ118232) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ME3 antibody (505-604) (STJ118232)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of RAW264.7 cells using ME3 Polyclonal Antibody

Anti-ME3 antibody (545-594 aa) (STJ94060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MECR antibody (160-210 aa) (STJ195034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of MECR in paraffin-embedded Human lung adenocarcinoma tissue using MECR Rabbit polyclonal antibody (STJ11105571) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-MECR antibody (54-373) (STJ11105571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

€138.13 - €631.88

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Anti-MECR antibody [SRM14958] (STJ11109688)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of extracts of MCF7 cells, using MED1 antibody (STJ24540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MED1 antibody (1-300) (STJ24540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Western blot analysis of various cells using TRAP220 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MED1 antibody (1423-1472 aa) (STJ96090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of various cells using TRAP220 Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-MED1 antibody (641-690 aa) (STJ96089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MED1 (PTR1203) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: DaudiPredicted band size: 220kDaObserved band size: 220kDa

Anti-MED1 antibody (650-750) [PTR1203] (STJA0006273)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

€61.25 - €326.25

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Anti-MED10 antibody (40-120 aa) (STJ190943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Anti-MED11 antibody (30-110 aa) (STJ190944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using MED12 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MED12 antibody (611-660 aa) (STJ96091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using MED13 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-MED13 antibody (681-730 aa) (STJ96092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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