Anti-HADHA antibody (545-763 aa) [SRM5146] (STJ11106984)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal HADHA (545-763 aa) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5146 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
Target Information
| Gene Symbol | HADHA |
| Gene ID | 3030 |
| Uniprot ID | ECHA_HUMAN |
| Immunogen Region | 545-763 aa |
| Immunogen Sequence | FYTTRCLAPMMSEVIRILQE GVDPKKLDSLTTSFGFPVGA ATLVDEVGVDVAKHVAEDLG KVFGERFGGGNPELLTQMVS KGFLGRKSGKGFYIYQEGVK RKDLNSDMDSILASLKLPPK SEVSSDEDIQFRLVTRFVNE AVMCLQEGILATPAEGDIGA VFGLGFPPCLGGPFRFVDLY GAQKIVDRLKKYEAAYGKQF TPCQLLADHANSPNKKFYQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 545-763 of human HADHA (NP_000173.2). |
Additional Info
| Function | Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity. Independently of subunit beta, HADHA also exhibits a cardiolipin acyltransferase activity that participates in cardiolipin remodeling.cardiolipin is a major mitochondrial membrane phospholipid. HADHA may act downstream of Tafazzin/TAZ, that remodels monolysocardiolipin (MLCL) to a cardiolipin intermediate, and then HADHA may continue to remodel this species into mature tetralinoleoyl-cardiolipin. Has also been proposed to act directly on MLCL.capable of acylating MLCL using different acyl-CoA substrates, with highest activity for oleoyl-CoA. |
| Protein Name | Trifunctional Enzyme Subunit Alpha - Mitochondrial78 Kda Gastrin-Binding ProteinMonolysocardiolipin AcyltransferaseMlcl AtTp-Alpha Includes - Long-Chain Enoyl-Coa Hydratase - Long Chain 3-Hydroxyacyl-Coa Dehydrogenase |
| Database Links | Reactome: R-HSA-1482798Reactome: R-HSA-77285Reactome: R-HSA-77288Reactome: R-HSA-77305Reactome: R-HSA-77310Reactome: R-HSA-77346Reactome: R-HSA-77348Reactome: R-HSA-77350 |
| Cellular Localisation | MitochondrionMitochondrion Inner MembraneProtein Stability And Association With Mitochondrion Inner Membrane Do Not Require Hadhb |
| Alternative Antibody Names | Anti-Trifunctional Enzyme Subunit Alpha - Mitochondrial antibodyAnti-78 Kda Gastrin-Binding Protein antibodyAnti-Monolysocardiolipin Acyltransferase antibodyAnti-Mlcl At antibodyAnti-Tp-Alpha Includes - Long-Chain Enoyl-Coa Hydratase - Long Chain 3-Hydroxyacyl-Coa Dehydrogenase antibodyAnti-HADHA antibodyAnti-HADH antibody |
Information sourced from Uniprot.org