Anti-HAS2 antibody (67-185) (STJ111939)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HAS2 (67-185) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HAS2 |
| Gene ID | 3037 |
| Uniprot ID | HYAS2_HUMAN |
| Immunogen Region | 67-185 |
| Immunogen Sequence | EHRKMKKSLETPIKLNKTVA LCIAAYQEDPDYLRKCLQSV KRLTYPGIKVVMVIDGNSED DLYMMDIFSEVMGRDKSATY IWKNNFHEKGPGETDESHKE SSQHVTQLVLSNKSICIMQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 67-186 of human HAS2 (NP_005319.1). |
Additional Info
| Tissue Specificity | Expressed in fibroblasts. |
| Post Translational Modifications | Phosphorylation at Thr-328 is essential for hyaluronan synthase activity. Phosphorylation at Thr-110 is required for transport from ER to Golgi. O-GlcNAcylation at Ser-221 increases the stability of HAS2 and plasma membrane localization. Ubiquitination at Lys-190.this ubiquitination is essential for hyaluronan synthase activity and homo- or hetero-oligomerization. Can also be poly-ubiquitinated. Deubiquitinated by USP17 and USP4. USP17 efficiently removes 'Lys-63'- and 'Lys-48'-linked polyubiquitin chains, whereas USP4 preferentially removes monoubiquitination and, partially, both 'Lys-63'- and 'Lys-48'-linked polyubiquitin chain. |
| Function | Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer (Probable). Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis and it is particularly responsible for the synthesis of high molecular mass hyaluronan. |
| Protein Name | Hyaluronan Synthase 2Hyaluronate Synthase 2Hyaluronic Acid Synthase 2Ha Synthase 2 |
| Database Links | Reactome: R-HSA-2142850 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEndoplasmic Reticulum MembraneVesicleGolgi Apparatus MembraneLysosomeTravels From Endoplasmic Reticulum (Er)Golgi To Plasma Membrane And Either Back To Endosomes And LysosomesOr Out Into Extracellular VesiclesPost-Translational Modifications Control Has2 Trafficking |
| Alternative Antibody Names | Anti-Hyaluronan Synthase 2 antibodyAnti-Hyaluronate Synthase 2 antibodyAnti-Hyaluronic Acid Synthase 2 antibodyAnti-Ha Synthase 2 antibodyAnti-HAS2 antibody |
Information sourced from Uniprot.org