• Immunofluorescence analysis of U-2 OS cells using HCCS Polyclonal Antibody (STJ29626) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using HCCS antibody (STJ29626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HCCS antibody (1-268) (STJ29626)

SKU:
STJ29626

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCCS (1-268) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HCCS
Gene ID: 3052
Uniprot ID: CCHL_HUMAN
Immunogen Region: 1-268
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human HCCS (NP_005324.3).
Immunogen Sequence: MGLSPSAPAVAVQASNASAS PPSGCPMHEGKMKGCPVNTE PSGPTCEKKTYSVPAHQERA YEYVECPIRGTAAENKENLD PSNLMPPPNQTPAPDQPFAL STVREESSIPRADSEKKWVY PSEQMFWNAMLKKGWKWKDE DISQKDMYNIIRIHNQNNEQ AWKEILKWEALHAAECPCGP SLIRFGGKAKEYSPRARIRS WMGYELPFDRHDWIINRCGT EVRYVIDYYDGGEVNKDYQ
Function Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.
Protein Name Holocytochrome C-Type Synthase
Cytochrome C-Type Heme Lyase
Cellular Localisation Mitochondrion Inner Membrane
Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Holocytochrome C-Type Synthase antibody
Anti-Cytochrome C-Type Heme Lyase antibody
Anti-HCCS antibody
Anti-CCHL antibody

Information sourced from Uniprot.org

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