Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Cleaved PARP1 p25 antibody (STJ11101731) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PARP1 antibody (150-214) [S1MR] (STJ11101731)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

€148.13 - €651.88

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Zhou, Li, et al. "Coptisine induces apoptosis in human hepatoma cells through activating 67-kDa laminin receptor/cGMP signaling. " Frontiers in pharmacology 9 (2018).

Anti-PARP1 antibody (410-460 aa) (STJ193190)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of extracts of various cell lines, using PARP1 antibody (STJ114859) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PARP1 antibody (662-1014) (STJ114859)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €773.13

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Western blot analysis of extracts of Jurkat cells, using PARP1 antibody (STJ11101720) at 1:1000 dilution. Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PARP1 antibody (700-800) [S0MR] (STJ11101720)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€179.38 - €773.13

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Confocal immunofluorescence analysis of U-2 OS cells using PARP1 Rabbit polyclonal antibody (STJ29827) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-PARP1 antibody (81-390) (STJ29827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis using PARP-1 monoclonal antibody against Jurkat (1) , K562 (2) , HeLa (3) , Raji (4) , THP-1 (5) and SW620 (6) cell lysate.

Anti-PARP1 antibody [7A10] (STJ98308)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/ELISA

Reactivity :

Human

€186.25 - €450.00

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Western blot analysis of 1) Hela, 2) 293T, 3) Jurkat, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PARP1 antibody [M3] (STJ97033)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Chicken

€61.25 - €326.25

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Anti-PARP1 antibody [SRM15025] (STJ11109755)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from rat brain, using PARS2 Rabbit polyclonal antibody (STJ118940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PARS2 antibody (30-220) (STJ118940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€138.13 - €631.88

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Immunofluorescence analysis of U-2 OS cells using PAX6 Rabbit polyclonal antibody (STJ29473) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PAX6 antibody (1-422) (STJ29473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of PAX6 in K562, Hela lysates using PAX6 antibody.

Anti-PAX6 antibody (190-310 aa) [R01-1G8] (STJA0010781)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€330.63

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Immunohistochemistry analysis of PAX6 in paraffin-embedded mouse pancreatic islet using PAX6 Rabbit monoclonal antibody (STJ11101636) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PAX6 antibody (323-422) [S6MR] (STJ11101636)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Anti-PAX6 antibody (STJA0010226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western Blot analysis using Pax-6 Monoclonal Antibody against recombinant Pax-6 protein (1).

Anti-PAX6 antibody [1C8] (STJ98310)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/ELISA

Reactivity :

Human

€186.25 - €450.00

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Anti-PAX6 antibody [PT0618R] (STJA0039384)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€187.50 - €675.00

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Anti-PAX6 antibody [SRM14718] (STJ11109448)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Anti-PC/aPC antibody [R41C17] (STJA0022419)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

€261.88 - €1,666.88

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Western blot analysis of extracts of various cell lines, using PCCB antibody (STJ27368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-PCCB antibody (260-539) (STJ27368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Anti-PCCB antibody (STJA0008383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-PCCB antibody [SRM14784] (STJ11109514)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PCGF2 antibody (STJ193132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells using 1 Mu g PCMT1 antibody (STJ28767). Western blot was performed from the immunoprecipitate using PCMT1 antibody (STJ28767) at a dilution of 1:1000.

Anti-PCMT1 antibody (62-286) (STJ28767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-PCMT1 antibody (STJA0007098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

€61.25 - €326.25

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TRAF6 regulates autophagy and apoptosis of melanoma cells through c-Jun/ATG16L2 signaling pathway. Cong Peng IHC mouse 1:350 SK-Mel-5 cell-xenograft

Anti-PCNA antibody (1-100 aa) [ABT221] (STJ197033)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€225.00 - €326.25

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Immunohistochemistry analysis of paraffin-embedded rat testis using PCNA Rabbit polyclonal antibody (STJ115299) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PCNA antibody (162-261) (STJ115299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunohistochemistry analysis of paraffin-embedded rat testis using PCNA Rabbit polyclonal antibody (STJ24918) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PCNA antibody (162-261) (STJ24918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-PCNA antibody (162-261) [S3MR] (STJ11101413)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/African green monkey

€179.38 - €773.13

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Western blot analysis of lysates from 1) HepG2, 2) HuvEC, 3) COS7 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-PCNA antibody (61-110 aa) (STJ94982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Rabbit

€61.25 - €326.25

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Anti-PCNA antibody (C-Term) (STJ13101593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

€414.38

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Formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of follicular center cells

Anti-PCNA antibody (Full length) [ZR378] (STJ180587)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

€201.88 - €666.88

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Western blot analysis of Hela cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour) cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PCNA antibody (Internal) (STJ98857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-PCNA antibody [12D10] {AbFluor™ 350} (STJA0006137)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {AbFluor™ 405} (STJA0006138)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {AbFluor™ 488} (STJA0006139)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {AbFluor™ 555} (STJA0006140)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {AbFluor™ 594} (STJA0006141)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {AbFluor™ 647} (STJA0006142)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {AbFluor™ 680} (STJA0006143)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {Biotin} (STJA0006144)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {Cy3} (STJA0006145)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {Cy5} (STJA0006146)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {FITC} (STJA0006147)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [12D10] {HRP} (STJA0006148)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse

€186.25 - €310.00

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Anti-PCNA antibody [1A041] (STJA0019917)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

€208.13 - €284.38

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Western blot detection of PCNA in Hela, 3T3, COS7, C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.

Anti-PCNA antibody [2E1-G10-H10] (STJ99203)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC/IHC

Reactivity :

Human/Mouse/Monkey/Rat/Hamster

€186.25 - €450.00

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Anti-PCNA antibody [9C6] {HRP} (STJA0042572)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

€161.88 - €251.88

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Anti-PCNA antibody [IHC711] (STJA0042240)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-p

Reactivity :

Human

€195.63 - €559.38

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Anti-PCNA antibody [PT0224R] (STJA0039630)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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