• Immunohistochemistry analysis of CD10/MME in paraffin-embedded human tonsil tissue using CD10/MME Rabbit polyclonal antibody (STJ27631) at a dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CD10/MME in paraffin-embedded human tonsil using CD10/MME Rabbit polyclonal antibody (STJ27631) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CD10/MME in paraffin-embedded human liver using CD10/MME Rabbit polyclonal antibody (STJ27631) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CD10/MME Rabbit polyclonal antibody (STJ27631) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MME antibody (52-352) (STJ27631)

SKU:
STJ27631

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MME (52-352) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MME
Gene ID: 4311
Uniprot ID: NEP_HUMAN
Immunogen Region: 52-352
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 52-352 of human CD10/MME (NP_000893.2).
Immunogen Sequence: YDDGICKSSDCIKSAARLIQ NMDATTEPCTDFFKYACGGW LKRNVIPETSSRYGNFDILR DELEVVLKDVLQEPKTEDIV AVQKAKALYRSCINESAIDS RGGEPLLKLLPDIYGWPVAT ENWEQKYGASWTAEKAIAQL NSKYGKKVLINLFVGTDDKN SVNHVIHIDQPRLGLPSRDY YECTGIYKEACTAYVDFMIS VARLIRQEERLPIDENQLAL EMNKVMELEKEIANATAKP
Post Translational Modifications Myristoylation is a determinant of membrane targeting. Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.
Function Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Protein Name Neprilysin
Atriopeptidase
Common Acute Lymphocytic Leukemia Antigen
Calla
Enkephalinase
Neutral Endopeptidase 24.11
Nep
Neutral Endopeptidase
Skin Fibroblast Elastase
Sfe
Cd Antigen Cd10
Database Links Reactome: R-HSA-2022377
Reactome: R-HSA-5578768
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Neprilysin antibody
Anti-Atriopeptidase antibody
Anti-Common Acute Lymphocytic Leukemia Antigen antibody
Anti-Calla antibody
Anti-Enkephalinase antibody
Anti-Neutral Endopeptidase 24.11 antibody
Anti-Nep antibody
Anti-Neutral Endopeptidase antibody
Anti-Skin Fibroblast Elastase antibody
Anti-Sfe antibody
Anti-Cd Antigen Cd10 antibody
Anti-MME antibody
Anti-EPN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance