Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of A549, K562 cells using Smad1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-SMAD1 antibody (150-230 aa) (STJ96712)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit polyclonal antibody (STJ11104508) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SMAD1 antibody (20-240) (STJ11104508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of extracts of C6 cells, using Smad1 antibody (STJ25602) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SMAD1 antibody (20-240) (STJ25602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Smad1/9 (PTR2351) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2Predicted band size: 52kDaObserved band size: 52kDa

Anti-SMAD1 antibody (400-465) [PTR2351] (STJA0006276)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Smad1 antibody [100] (STJA0032670)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

$309.81 - $425.73

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Anti-Smad1 antibody [PT0687R] (STJA0039763)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-Smad1 antibody [R02-1J1] (STJA0036149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human

$184.23 - $388.47

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Anti-Smad1 antibody [R03-4B4] (STJA0035864)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

$184.23 - $388.47

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Anti-Smad1 antibody [SRM13316] (STJ11108046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$0.00

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Anti-Smad1 antibody [SRM14414] (STJ11109144)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

$0.00

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Anti-Smad1 antibody [SRM14522] (STJ11109252)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

$0.00

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Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (STJ110012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-SMAD2 antibody (1-100) (STJ110012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Smad2 antibody (STJ11101651). Western blot was performed from the immunoprecipitate using Smad2 antibody (STJ11101651) at a dilution of 1:1000.

Anti-SMAD2 antibody (1-100) [S1MR] (STJ11101651)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunohistochemistry analysis of paraffin-embedded human tonsil using Smad2 Rabbit polyclonal antibody (STJ11103803) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD2 antibody (175-270) (STJ11103803)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of lysates from wild type (WT) and SMAD2 knockout (KO) Hela (KO) cells, using [KO Validated] Smad2 Rabbit monoclonal antibody (STJ11104898) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SMAD2 antibody (367-467 aa) [S4898RM] (STJ11104898)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Immunofluorescence analysis of Hela cell. 1, Smad2 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). MICU1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD2 antibody (418-467 aa) (STJ95694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/CoIP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunocytochemistry analysis of Smad2 (green) in Hela using Smad2 antibody, and DAPI (blue).

Anti-SMAD2 antibody (81-100 aa) [R03-3F8] (STJA0013074)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

$184.23 - $388.47

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Anti-SMAD2 antibody [3C4] (STJ16103493)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/FC

Reactivity :

Human

$1,030.17

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Western blot analysis using Smad2 monoclonal antibody against HEK293 (1) and SMAD2-hIgGFc transfected HEK293 (2) cell lysate.

Anti-SMAD2 antibody [5G7] (STJ98383)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$205.62 - $496.80

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Western blot detection of Smad2 in Hela, A431, Jurkat and K562 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-SMAD2 antibody [6H5-E3-C11] (STJ99174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

$205.62 - $496.80

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Anti-Smad2 antibody [6H5-E3-C11] (STJA0032427)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

$184.23 - $388.47

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Anti-Smad2 antibody [PT0111R] (STJA0039647)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Immunohistochemistry analysis of Smad3 in paraffin-embedded human tonsil using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD3 antibody (100-200) [S2MR] (STJ11101652)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Immunofluorescence analysis of Hela cell. 1, Smad3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-actin monoclonal antibody (5B7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD3 antibody (145-194 aa) (STJ95699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of NIH/3T3 cells using Smad3 Rabbit polyclonal antibody (STJ11101192) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SMAD3 antibody (150-425) (STJ11101192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunocytochemistry analysis of Smad3 (green) in A549 using Smad3 antibody, and DAPI (blue).

Anti-SMAD3 antibody (202-219 aa) [R09-7H4] (STJA0010795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

$184.23 - $388.47

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Immunohistochemical analysis of paraffin-embedded Human Liver Carcinoma Tissue using Smad3 Mouse mAb diluted at 1:200.

Anti-SMAD3 antibody (350-430 aa) [4C9] (STJ97772)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Smad3 antibody [4H4-10A10-7B4] (STJA0032967)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

$184.23 - $388.47

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Anti-Smad3 antibody [PT0830R] (STJA0039185)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$393.30 - $745.20

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Anti-Smad3 antibody [R02-1I2] (STJA0034867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Hamster

$184.23 - $388.47

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Anti-Smad3 antibody [SRM13315] (STJ11108045)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IHC-P/ELISA

Reactivity :

Human

$0.00

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Anti-Smad3 antibody [SRM14491] (STJ11109221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Human pancreatic ductal adenocarcinoma stained with anti-DPC4 (SMAD4) antibody using peroxidase-conjugate and DAB chromogen. Note absence of tumor cell staining while the non-tumor cells are positive.

Anti-SMAD4 antibody (1-552 aa) [B‐8] (STJ180086)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$203.55 - $779.01

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Immunohistochemistry of mouse Colon with Anti-SMAD4 primary antibody (STJ503059) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (131-181) (STJ503059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$552.69

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Anti-SMAD4 antibody (131-181) {Biotin} (STJ503063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-SMAD4 antibody (131-181) {FITC} (STJ503064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-SMAD4 antibody (136-156) {FITC} (STJ502007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

$686.55

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Immunofluorescence analysis of Hela cell. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD4 antibody (21-70 aa) (STJ95702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Immunocytochemistry analysis of Smad4 (green) in Hela using Smad4 antibody, and DAPI (blue).

Anti-SMAD4 antibody (316-552 aa) [R05-1I7] (STJA0012592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

$184.23 - $388.47

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Western blot analysis of lysates from HCT116 cells, using Smad4 Rabbit pAb (STJ27624) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-SMAD4 antibody (320-552) (STJ27624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g Smad4 rabbit monoclonal antibody (STJ11101653) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of Smad4 in representative gene loci (JUNB) , as shown in figure.

Anti-SMAD4 antibody (320-552) [S3MR] (STJ11101653)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Immunohistochemistry of mouse liver with Anti-SMAD4 primary antibody (STJ503060) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (400-450) (STJ503060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

$552.69

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Immunohistochemistry with Anti-SMAD4 Antibody (STJ503062) at 1:50 dilution in buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 20X magnification. FFPE section.

Anti-SMAD4 antibody (400-450) (STJ503062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Monkey/Mouse/Rat

$552.69

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Anti-SMAD4 antibody (400-450) {Biotin} (STJ503065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

$686.55

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Anti-SMAD4 antibody (400-450) {Biotin} (STJ503069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

$686.55

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Anti-SMAD4 antibody (400-450) {FITC} (STJ503066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

$686.55

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Anti-SMAD4 antibody (400-450) {FITC} (STJ503070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

$686.55

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Immunohistochemistry of mouse small intestine with Anti-SMAD4 primary antibody (STJ503061) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (502-552) (STJ503061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$552.69

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