Haemostasis | Research Area | St John's Laboratory

Immunofluorescence analysis of Hela cell. 1, Lck (phospho Tyr393) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-LCK-Tyr393 antibody (361-410 aa) (STJ90732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from Jurkat cells, using Lck (Phospho-Tyr505) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-LCK-Tyr505 antibody (460-509 aa) (STJ90820)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-LCK-Y394 Rabbit pAb (STJ22226) at 1:1000 dilution. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-LCK-Y394 antibody (STJ22226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€151.80 - €742.20

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Western blot analysis of lysates from Jurkat cells, using Phospho-Lck-Y505 Rabbit pAb (STJ22227) at 1:1000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37 °C for 2 minutes or treated by Pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1s.

Anti-Phospho-LCK-Y505 antibody (STJ22227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

€151.80 - €742.20

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-LCP2-Ser376 antibody (STJ196426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of K562 cells using Phospho-SLP-76 (Y128) Polyclonal Antibody

Anti-Phospho-LCP2-Tyr128 antibody (94-143 aa) (STJ90961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-LCP2-Tyr145 antibody (STJ196427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from U937 cells, using Phospho-LCP2-Y145 Rabbit pAb (STJ117914) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-LCP2-Y145 antibody (STJ117914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€151.80 - €742.20

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Anti-Phospho-Lyn antibody (425-525) (STJ502546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€597.00

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Anti-Phospho-Lyn antibody (425-525) {Biotin} (STJ502547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€661.80

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Anti-Phospho-Lyn antibody (425-525) {FITC} (STJ502548)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€661.80

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Phospho-Lyn antibody western blot Jurkat lysates STJA0036232

Anti-Phospho-Lyn-Tyr396 antibody [R07-3H2] (STJA0036232)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/FC

Reactivity :

Human/Mouse

€160.20 - €337.80

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Anti-Phospho-LYN-Tyr507 antibody (STJ196350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-Lyn (Y508) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-LYN-Tyr508 antibody (463-512 aa) (STJ90764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Lyn-Y396 Rabbit mAb (STJ11102620) at 1:1000 dilution. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-LYN-Y396 antibody [S0MR] (STJ11102620)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Anti-Phospho-MAPK1 antibody [M206] (STJA0005411)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€468.60

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Anti-Phospho-MAPK14 antibody (250-300) (STJ502555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€597.00

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Anti-Phospho-MAPK14 antibody (250-300) {Biotin} (STJ502556)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€661.80

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Anti-Phospho-MAPK14 antibody (250-300) {FITC} (STJ502557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

€661.80

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Immunohistochemistry analysis of paraffin-embedded rat ovary using Phospho-p38 MAPK-Y182 Rabbit polyclonal antibody (STJ11105887) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-MAPK14-T180 antibody (STJ11105887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180 antibody (147-196 aa) (STJ90362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Immunofluorescence analysis of Hela cell. 1, p38 (phospho Thr180/Y182) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-MAPK14-Thr180/Y182 antibody (151-200 aa) (STJ90498)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/FC/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Guinea Pig

€58.80 - €313.20

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Western blot analysis of lysates from 1) PC-3, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta monoclonal antibody (5G3) (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Phospho-MAPK14-Tyr182 antibody (147-196 aa) (STJ90363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of human-stomach tissue. 1, p38 (phospho Tyr323) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-MAPK14-Tyr323 antibody (288-337 aa) (STJ90878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-Y182 Rabbit pAb (STJ22257) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-Phospho-MAPK14-Y182 antibody (STJ22257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ERK1-Y204 + ERK2-Y187 Rabbit pAb (STJ22262) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-MAPK3-Y204 antibody (STJ22262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot MerTK phosphorylation in HEK293 lysate with lambda phosphatase treatment STJA0004902

Anti-Phospho-MerTK-Tyr749/753/754 antibody (STJA0004902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Finch/Guinea Pig/Non-Human Primates

€586.20

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Anti-Phospho-MPL-Tyr626 antibody (STJ196457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Phospho-v-Myb c-Myb antibody western blot Ramos STJA0035662

Anti-Phospho-Myb-Ser11 antibody [R11-7D-7] (STJA0035662)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

€160.20 - €337.80

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Immunohistochemistry analysis of paraffin-embedded human heart, using MYB (Phospho-Ser12) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MYB-Ser12 antibody (1-50 aa) (STJ90821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Myb (Phospho-Ser532) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-MYB-Ser532 antibody (496-545 aa) (STJ91262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using n-NOS (Phospho-Ser852) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NOS1-Ser852 antibody (818-867 aa) (STJ90355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Anti-Phospho-NOS2-Tyr-1055 antibody (STJA0005496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€441.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using iNOS (Phospho-Tyr151) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NOS2-Tyr151 antibody (117-166 aa) (STJ91227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-eNOS-S1177 Rabbit pAb (STJ110855) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Phospho-NOS3-S1177 antibody (STJ110855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

€151.80 - €742.20

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Western blot analysis of lysates from Jurkat cells, using Phospho-eNOS-S1177 Rabbit pAb (STJ22320). Jurkat cells were treated by Calyculin A. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-NOS3-S1177 antibody (STJ22320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€151.80 - €742.20

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Western blot analysis of 293T lysates, using Phospho-eNOS-S1177 antibody (STJ11105869) at 1:7000 dilution. 293T cells were treated by IGF-1 (50 ng/ml) at 37 °C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-NOS3-S1177 antibody [S5869RM] (STJ11105869)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€142.20 - €625.80

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Anti-Phospho-NOS3-Ser-1177 antibody (STJA0005493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€441.00

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Western Blot analysis of 1 HEK-293 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NOS3-Ser114 antibody (STJA0010055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of Hela cell. 1, NOS3 (phospho Ser1177) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-NOS3-Ser1177 antibody (1144-1193 aa) (STJ90674)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western Blot analysis of K562 cells, cell treated or untreated by EGF 20ng/ml 30'. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-Phospho-NOS3-Ser1179 antibody (STJ193218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from K562 cells treated with EGF 40nM 30', using eNOS (Phospho-Ser615) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-NOS3-Ser615 antibody (581-630 aa) (STJ90541)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western Blot analysis of mouse heart, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NOS3-Ser633 antibody (STJA0009921)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of 293 cells using Phospho-NOS3 (T494) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-NOS3-Thr494 antibody (462-511 aa) (STJ90356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-NOS3-Tyr-657 antibody (STJA0005492)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€441.00

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Western blot analysis of lysates from HeLa cells, using Phospho-MAPK14-T180/Y182 pAb (STJ11107626) at 1:2000 dilution or MAPK14 antibody (STJ228561). HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-p38 MAPK-T180/Y182 antibody (STJ11107626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-p38 MAPK-Y182-Y182 antibody [SRM15123] (STJ11109853)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Anti-Phospho-p38 MAPK-Y182-Y182 antibody [SRM15147] (STJ11109877)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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