• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-LCK-Y394 antibody (STJ22226) at 1:1000 dilution. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of Raji pervanadate and Raji cells using Phospho-LCK-Y394 Rabbit polyclonal antibody (STJ22226) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Jurkat cells, using Phospho-LCK-Y394 antibody (STJ22226) at 1:1000 dilution. Jurkat cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of lysates from Jurkat cells, using Phospho-LCK-Y394 Rabbit polyclonal antibody (STJ22226) at 1:1000 dilution. Jurkat cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-LCK-Y394 Rabbit polyclonal antibody (STJ22226) at 1:1000 dilution. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-LCK-Y394 antibody (STJ22226)

SKU:
STJ22226

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-LCK-Y394 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LCK
Gene ID: 3932
Uniprot ID: LCK_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y394 of human LCK (NP_005347.3).
Immunogen Sequence: NEYTA
Tissue Specificity Expressed specifically in lymphoid cells.
Post Translational Modifications Autophosphorylated on Tyr-394, increasing enzymatic activity, this site is dephosphorylated by PTN22. Phosphorylated on Tyr-505 by CSK, decreasing activity. Dephosphorylated by PTPRC/CD45. Dephosphorylation at Tyr-394 by PTPN2 negatively regulates T-cell receptor signaling. Myristoylation is required prior to palmitoylation. Palmitoylation regulates association with the plasma membrane and could be mediated by ZDHHC2.
Function Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2.
Protein Name Tyrosine-Protein Kinase Lck
Leukocyte C-Terminal Src Kinase
Lsk
Lymphocyte Cell-Specific Protein-Tyrosine Kinase
Protein Yt16
Proto-Oncogene Lck
T Cell-Specific Protein-Tyrosine Kinase
P56-Lck
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1257604
Reactome: R-HSA-1433557
Reactome: R-HSA-1433559
Reactome: R-HSA-164944
Reactome: R-HSA-167590
Reactome: R-HSA-202424
Reactome: R-HSA-202427
Reactome: R-HSA-202430
Reactome: R-HSA-202433
Reactome: R-HSA-210990
Reactome: R-HSA-2219530
Reactome: R-HSA-2424491
Reactome: R-HSA-389356
Reactome: R-HSA-389357
Reactome: R-HSA-389359
Reactome: R-HSA-389513
Reactome: R-HSA-389948
Reactome: R-HSA-6811558
Reactome: R-HSA-9013407
Reactome: R-HSA-9020558
Reactome: R-HSA-9670439
Reactome: R-HSA-9706374
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cytoplasm
Cytosol
Present In Lipid Rafts In An Inactive Form
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Lck antibody
Anti-Leukocyte C-Terminal Src Kinase antibody
Anti-Lsk antibody
Anti-Lymphocyte Cell-Specific Protein-Tyrosine Kinase antibody
Anti-Protein Yt16 antibody
Anti-Proto-Oncogene Lck antibody
Anti-T Cell-Specific Protein-Tyrosine Kinase antibody
Anti-P56-Lck antibody
Anti-LCK antibody

Information sourced from Uniprot.org

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