• Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using eNOS (Phospho-Ser1176) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1：1000
  • Western blot analysis of HuvEc cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1：1000
  • Immunofluorescence analysis of Hela cell. 1, NOS3 (phospho Ser1177) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-NOS3-Ser1177 antibody (1144-1193 aa) (STJ90674)

SKU:
STJ90674

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Nitric oxide synthase 3-Ser1177 (1144-1193 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOS3
Gene ID: 4846
Uniprot ID: NOS3_HUMAN
Immunogen Region: 1144-1193 aa
Specificity: Phospho-NOS3 (S1177) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at S1177.
Immunogen: The antiserum was produced against synthesized peptide derived from the human eNOS around the phosphorylation site of Ser1176 at the amino acid range 1144-1193
Post Translational Modifications Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity.
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Protein Name Nitric Oxide Synthase 3
Constitutive Nos
Cnos
Ec-Nos
Nos Type Iii
Nosiii
Nitric Oxide Synthase - Endothelial
Endothelial Nos
Enos
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1474151
Reactome: R-HSA-203615
Reactome: R-HSA-203641
Reactome: R-HSA-203754
Reactome: R-HSA-392154
Reactome: R-HSA-5218920
Reactome: R-HSA-9009391
Cellular Localisation Cell Membrane
Membrane
Caveola
Cytoplasm
Cytoskeleton
Golgi Apparatus
Specifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell Cycle
Which Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity
Alternative Antibody Names Anti-Nitric Oxide Synthase 3 antibody
Anti-Constitutive Nos antibody
Anti-Cnos antibody
Anti-Ec-Nos antibody
Anti-Nos Type Iii antibody
Anti-Nosiii antibody
Anti-Nitric Oxide Synthase - Endothelial antibody
Anti-Endothelial Nos antibody
Anti-Enos antibody
Anti-NOS3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance