Anti-Phospho-LCP2-Tyr145 antibody (STJ196427)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phosho peptide around human SLP-76 (Tyr145)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Lymphocyte cytosolic protein 2-Tyr145 for use in WB and IHC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LCP2 |
| Gene ID | 3937 |
| Uniprot ID | LCP2_HUMAN |
| Immunogen | Synthesized phosho peptide around human SLP-76 (Tyr145) |
| Specificity | This antibody detects endogenous levels of Human Mouse SLP-76 (phospho-Tyr145) |
Additional Info
| Post Translational Modifications | Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components. |
| Function | Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells. Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK. In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation. Also plays an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses. |
| Protein Name | Lymphocyte Cytosolic Protein 2Sh2 Domain-Containing Leukocyte Protein Of 76 KdaSlp-76 Tyrosine PhosphoproteinSlp76 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-202433Reactome: R-HSA-2424491Reactome: R-HSA-2871796Reactome: R-HSA-2871809 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Lymphocyte Cytosolic Protein 2 antibodyAnti-Sh2 Domain-Containing Leukocyte Protein Of 76 Kda antibodyAnti-Slp-76 Tyrosine Phosphoprotein antibodyAnti-Slp76 antibodyAnti-LCP2 antibody |
Information sourced from Uniprot.org