• Western blot analysis of extracts of U937 cells, using Phospho-LCP2-Y145 antibody (STJ117914) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from U937 cells, using Phospho-LCP2-Y145 Rabbit polyclonal antibody (STJ117914) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-LCP2-Y145 antibody (STJ117914)

SKU:
STJ117914

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-LCP2-Y145 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LCP2
Gene ID: 3937
Uniprot ID: LCP2_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y145 of human LCP2 (NP_005556.1).
Immunogen Sequence: ADYEP
Tissue Specificity Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
Post Translational Modifications Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.
Function Involved in T-cell antigen receptor mediated signaling.
Protein Name Lymphocyte Cytosolic Protein 2
Sh2 Domain-Containing Leukocyte Protein Of 76 Kda
Slp-76 Tyrosine Phosphoprotein
Slp76
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-202433
Reactome: R-HSA-2424491
Reactome: R-HSA-2871796
Reactome: R-HSA-2871809
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Lymphocyte Cytosolic Protein 2 antibody
Anti-Sh2 Domain-Containing Leukocyte Protein Of 76 Kda antibody
Anti-Slp-76 Tyrosine Phosphoprotein antibody
Anti-Slp76 antibody
Anti-LCP2 antibody

Information sourced from Uniprot.org

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